An individual variation study of Electroacupuncture analgesia in rats using Microarray
An individual variation study of Electroacupuncture analgesia in rats using Microarray
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DOI：
10.1142/s0192415x07005259
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发表时间：
2007-01-01
影响因子：
5.7
通讯作者：
Jiang, Xing-Hong
中科院分区：
文献类型：
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作者：
Gao, Yu-Zhen;Guo, Shi-Yu;Jiang, Xing-Hong
本研究的目的是探讨与大鼠电针（EA）镇痛相关的候选基因，了解电针镇痛个体差异的分子基础。我们使用寡核苷酸微阵列比较了 ST36 穴位 1 Hz EA 治疗 1 小时后有反应者与无反应者的下丘脑转录谱。通过甩尾潜伏期（TFL）确定有反应者和无反应者。应用实时定量RT-PCR来验证差异表达基因。我们的研究提供了全局下丘脑转录亲。大鼠 EA 镇痛的 le。我们发现应答组中分别有 63 个和 3 个基因上调和下调。一半的差异表达基因分为9个功能组，分别是离子转运、感觉知觉、突触发生和突触传递、信号转导、炎症反应、细胞凋亡、转录、蛋白质氨基酸磷酸化和G蛋白信号传导。谷氨酸能受体、生长素释放肽前体、黑皮质素 4 受体 (MC4-R) 和神经肽 1 在反应组中被发现上调，这可能成为伤害性研究的新目标，值得进一步研究，以开发新的针灸疗法和疼痛调节干预。
The aim of the present study is to probe candidate genes which were involved in the electroacupuncture (EA) analgesia and to understand the molecular basis of the individual difference of EA analgesia in rats. We compared hypothalamus transcriptional profiles of responders with those of non-responders after 1 Hz EA treatment at ST36 acupoint for 1 hour by using oligonucleotide microarray. Responders and non-responders were determined by tail flick latency (TFL). A real-time quantitative RT-PCR was applied to validate the differential expressed genes. Our study provided a global hypothalamus transcriptional pro. le of EA analgesia in rats. We found that 63 and 3 genes were up- and down-regulated in the responder group, respectively. Half of the differentially expressed genes were classified into 9 functional groups which were ion transport, sensory perception, synaptogenesis and synaptic transmission, signal transduction, inflammatory response, apoptosis, transcription, protein amino acid phosphorylation and G-protein signaling. Glutamatergic receptors, ghrelin precursor, melanocortin 4 receptor (MC4-R) and neuroligin 1 were found to be up- regulated in the responder group which may become new targets for nociceptive study and deserve further investigation for developing new acupuncture therapy and intervention of pain modulation.