The two subunits of a phospholipase A2 inhibitor from the plasma of Thailand cobra having structural similarity to urokinase-type plasminogen activator receptor and LY-6 related proteins.
The two subunits of a phospholipase A2 inhibitor from the plasma of Thailand cobra having structural similarity to urokinase-type plasminogen activator receptor and LY-6 related proteins.
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来自泰国眼镜蛇血浆的磷脂酶A2抑制剂的两个亚基与尿激酶型纤溶酶原激活剂受体和LY-6相关蛋白具有结构相似性。
DOI：
10.1006/bbrc.1994.2592
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发表时间：
1994
影响因子：
3.1
通讯作者：
K. Hayashi
中科院分区：
文献类型：
--
作者：
N. Ohkura;S. Inoue;K. Ikeda;K. Hayashi
从泰国眼镜蛇（Naja naja kaouthia）血浆中纯化的磷脂酶A2（PLA 2）抑制蛋白的两个亚基（31-kDa和25-kDa亚基）的氨基酸序列通过由赖氨酰内肽酶、葡萄球菌V8蛋白酶和内蛋白酶Asp-N diglysine获得的肽的比对来确定。相应的亚基由188和185个氨基酸残基组成，并且前者在位置157处含有一个天冬酰胺连接的糖链。31-kDa和25-kDa亚基之间有29%的同一性。两个亚基的每个序列中的内部同源性分析揭示了存在两个约90个氨基酸残基的重复。发现这些序列单位与尿激酶型纤溶酶原激活物受体和Ly-6相关蛋白如Ly-6A/E、Ly-6C、ThB和CD 59抗原的序列单位显著同源。
Amino acid sequences of the two subunits (31-kDa and 25-kDa subunits) of a phospholipase A2 (PLA2) inhibitory protein, purified from the blood plasma of Thailand cobra Naja naja kaouthia, were determined by alignment of peptides obtained by lysyl endopeptidase, staphylococcal V8 protease and endoproteinase Asp-N digestions. The respective subunits were composed of 188 and 185 amino acid residues, and the former contained one asparagine-linked sugar chain at the position 157. There was 29% identity between 31-kDa and 25-kDa subunits. The analysis of internal homology in each sequence of the two subunits revealed the existence of two repeats of approximately 90 amino acid residues. These sequence units were found to be significantly homologous to those of urokinase-type plasminogen activator receptor and of Ly-6 related proteins, such as Ly-6A/E, Ly-6C, ThB, and CD59 antigens.