Atomic Force Microscopy (AFM) Applications in Arrhythmogenic Cardiomyopathy.
Atomic Force Microscopy (AFM) Applications in Arrhythmogenic Cardiomyopathy.
复制标题
原子力显微镜在致心律失常性心肌病中的应用。
DOI：
10.3390/ijms23073700
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发表时间：
2022-03-28
影响因子：
5.6
通讯作者：
Sbaizero O
中科院分区：
文献类型：
--
作者：
Peña B;Adbel-Hafiz M;Cavasin M;Mestroni L;Sbaizero O
致心律失常性心肌病（ACM）是一种遗传性心肌疾病，其特征是心肌细胞逐渐被纤维脂肪组织替代、心室扩张、心功能障碍、心律失常和心源性猝死。对这些疾病的分子生物力学的兴趣正在不断增长。原子力显微镜（AFM）是一种成熟的技术，用于在细胞尺度上研究生理和病理条件下生物样品的机械生物学。然而，描述所有不同的数据，可以使用AFM（细胞弹性，粘附行为，粘弹性，打浆力和频率）的评论仍然缺失。在这篇综述中，我们将讨论几种技术，突出的潜力，原子力显微镜被用作评估生物力学参与ACM的工具。事实上，用AFM分析基因突变的细胞揭示了细胞骨架、细胞膜结构和收缩性缺陷的异常。杨氏模量越高，细胞越硬，并且众所周知，异常的组织硬度是一系列疾病的症状。细胞搏动力和频率在去极化和复极化阶段提供信息，补充细胞电生理学（钙成像，MEA，膜片钳）。此外，原始数据也强调了AFM作为评估心脏组织纤维化的工具的独特潜力。
Arrhythmogenic cardiomyopathy (ACM) is an inherited heart muscle disorder characterized by progressive replacement of cardiomyocytes by fibrofatty tissue, ventricular dilatation, cardiac dysfunction, arrhythmias, and sudden cardiac death. Interest in molecular biomechanics for these disorders is constantly growing. Atomic force microscopy (AFM) is a well-established technic to study the mechanobiology of biological samples under physiological and pathological conditions at the cellular scale. However, a review which described all the different data that can be obtained using the AFM (cell elasticity, adhesion behavior, viscoelasticity, beating force, and frequency) is still missing. In this review, we will discuss several techniques that highlight the potential of AFM to be used as a tool for assessing the biomechanics involved in ACM. Indeed, analysis of genetically mutated cells with AFM reveal abnormalities of the cytoskeleton, cell membrane structures, and defects of contractility. The higher the Young’s modulus, the stiffer the cell, and it is well known that abnormal tissue stiffness is symptomatic of a range of diseases. The cell beating force and frequency provide information during the depolarization and repolarization phases, complementary to cell electrophysiology (calcium imaging, MEA, patch clamp). In addition, original data is also presented to emphasize the unique potential of AFM as a tool to assess fibrosis in cardiac tissue.
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影响因子：
4.6
作者：
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通讯作者：
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Bilder, David
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作者：
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通讯作者：
Peyronnet R