The protective effect of royal jelly against cisplatin-induced renal oxidative stress in rats
The protective effect of royal jelly against cisplatin-induced renal oxidative stress in rats
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DOI：
10.1007/s00345-010-0543-5
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发表时间：
2011-02-01
影响因子：
3.4
通讯作者：
Deniz, Kemal
中科院分区：
文献类型：
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作者：
Silici, Sibel;Ekmekcioglu, Oguz;Deniz, Kemal
本研究旨在探讨蜂王浆对顺铂所致大鼠肾毒性和氧化应激的影响。成年雄性Wistar白化大鼠随机分为8组：对照组、顺铂组、蜂王浆组、蜂王浆+顺铂组。采用生化和组织病理学方法评价肾毒性。收集血液并分析血尿素氮（BUN）、丙氨酸转氨酶、天冬氨酸转氨酶、甘油三酯、总胆固醇、尿酸、总胆红素和总蛋白质水平。保存肾脏样本用于测量丙二醛（MDA）、谷胱甘肽过氧化物酶（GSHPx）、超氧化物歧化酶（SOD）和过氧化氢酶（CAT）活性，并进行组织病理学检查。给予顺铂诱导大鼠明显肾衰竭，其特征是血清BUN和尿酸浓度显着升高，并且其肾脏MDA较高，GSH-Px、SOD和CAT活性较低。在服用 RJ 和 CP 的组中，观察到一些氧化应激参数和某些其他生化参数有所改善，RJ 预处理更有效。蜂王浆治疗部分逆转了 CP 引起的肾脏组织病理学变化。这些结果进一步深入了解了 CP 诱导的肾毒性机制，并证实了蜂王浆的抗氧化潜力。
The aim of this study was to investigate the effects of royal jelly on cisplatin-induced nephrotoxicity and oxidative stress in rats.Adult male Wistar albino rats were randomly divided into eight groups: the control, cisplatin, royal jelly, and royal jelly plus cisplatin groups. Biochemical and histopathological methods were utilized for evaluation of the nephrotoxicity. Blood was collected and analyzed for blood urea nitrogen (BUN), alanine aminotransferase, aspartate aminotransferase, triglyceride, total cholesterol, uric acid, total bilirubin, and total protein levels. The kidney samples were stored for the measurement of malondialdehyde (MDA), glutathione peroxidase (GSHPx), superoxide dismutase (SOD), and catalase (CAT) activities and processed for histopathological examinations.Administration of cisplatin to rats induced a marked renal failure, characterized with a significant increase in serum BUN and uric acid concentrations, and they had higher kidney MDA and lower GSH-Px, SOD, and CAT activities. In the groups that were administered RJ in association with CP, improvement was observed in some oxidative stress parameters and certain other biochemical parameters, pre-treatment with RJ being more effective.The CP-induced changes in histopathologic findings of kidneys were partially reversed by treatment with royal jelly. The results provide further insight into the mechanisms of CP-induced nephrotoxicity and confirm the antioxidant potential of royal jelly.