Influence of dietary fatty acid concentration and geometric configuration on murine mammary tumorigenesis and experimental metastasis.
Influence of dietary fatty acid concentration and geometric configuration on murine mammary tumorigenesis and experimental metastasis.
复制标题
膳食脂肪酸浓度和几何构型对小鼠乳腺肿瘤发生和实验转移的影响。
DOI：
10.1093/jn/114.10.1834
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
Judith S. Stern
中科院分区：
文献类型：
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作者：
K. Erickson;Deborah S. Schlanger;Dorothy A. Adams;David R. Fregeau;Judith S. Stern
为了研究脂肪酸几何异构体对乳腺肿瘤生长和实验性转移的影响，给小鼠喂食含有高顺式或反式脂肪酸脂肪的饮食。顺式脂肪被制备成具有与反式脂肪相似的脂肪酸组成；两者均以饮食的 5% 或 20%（按重量计）提供。将168系乳腺肿瘤细胞移植：1）皮下移植至雌性BALB/c小鼠体内，观察膳食脂肪对潜伏期和局部肿瘤生长的影响；2）静脉注射，观察对实验性转移的影响。在喂食含有顺式或反式脂肪酸的饮食的组中，没有观察到原发性肿瘤生长的潜伏期或速率存在差异。此外，在喂食实验饮食的各组中，除了原代肿瘤细胞中的反式C18:1水平外，脂肪酸组成没有差异。饲喂 5% 和 20% 顺式饮食的动物的肝脏和脾脏比饲喂反式饮食的动物含有明显更多的活放射性标记肿瘤细胞。尽管各组之间的体重和成分没有显着差异，但饲喂含有反式脂肪酸的饮食的动物的肝脏明显比饲喂仅含有顺式脂肪酸的饮食的动物重。因此，反式脂肪酸在促进可移植性乳腺肿瘤生长方面与顺式脂肪酸相似，但在促进肿瘤细胞的血源性植入和远处存活方面，反式脂肪酸不如顺式脂肪酸有效。
To investigate the influence of fatty acid geometric isomers on the growth and experimental metastasis of mammary tumors, mice were fed diets containing fat high in either cis or trans fatty acids. The cis fat was prepared to have a fatty acid composition similar to that of the trans fat; both were provided at 5 or 20% (by weight) of the diet. Line 168 mammary tumor cells were transplanted: 1) subcutaneously into female BALB/c mice to observe the effects of dietary fat on latency and local tumor growth, and 2) intravenously to observe influences on experimental metastasis. No differences in the latency or rate of primary tumor growth were observed among the groups fed the diets containing cis or trans fatty acids. In addition, there were no differences in fatty acid composition except the levels of trans-C18:1 in the primary tumor cells among the groups fed the experimental diets. Livers and spleens from animals fed both the 5 and 20% cis diet contained significantly more viable radiolabeled tumor cells than those fed the trans diets. Although body weight and composition were not significantly different among the groups, livers from animals fed the diets containing trans fatty acids were significantly heavier than those fed diets containing only cis fatty acids. Thus, trans fatty acids behaved similarly to cis fatty acids with respect to promotion of transplantable mammary tumor growth but trans fatty acids were less effective than cis fatty acids in promoting the blood-borne implantation and distant survival of the tumor cells.