Difference in bacterial motion between forward and backward swimming caused by the wall effect
Difference in bacterial motion between forward and backward swimming caused by the wall effect
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DOI：
10.1529/biophsj.104.054049
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发表时间：
2005-05-01
影响因子：
3.4
通讯作者：
Goto, T
中科院分区：
文献类型：
--
作者：
Magariyama, Y;Ichiba, M;Goto, T
具有单极鞭毛的细菌细胞交替地重复向前游动和向后游动，在向前游动中鞭毛推动细胞体，在向后游动中鞭毛拉动细胞体。我们已经报道了溶藻弧菌向后游泳的速度平均大于向前游泳的速度。在这项研究中，我们定量测量的轨迹的形状以及游泳速度。前向模式下的弹道形状几乎是直的，而后向模式下的弹道形状是弯曲的。在离表面不同的距离处测量相同的参数。当细胞在表面附近游动时，游动模式之间的运动特性的差异是显著的。相比之下，当细胞游离任何表面>60 μ m时，差异就难以区分。此外，一个细胞在向后模式往往停留在表面附近的时间比一个细胞在向前模式。这种壁面效应对细菌运动的影响与化学修饰无关。玻璃表面的阳离子。在实验结果的基础上对宏观行为进行了数值模拟，并讨论了这一现象的意义。
A bacterial cell that has a single polar flagellum alternately repeats forward swimming, in which the flagellum pushes the cell body, and backward swimming, in which the flagellum pulls the cell body. We have reported that the backward swimming speeds of Vibrio alginolyticus are on average greater than the forward swimming speeds. In this study, we quantitatively measured the shape of the trajectory as well as the swimming speed. The trajectory shape in the forward mode was almost straight, whereas that in the backward mode was curved. The same parameters were measured at different distances from a surface. The difference in the motion characteristics between swimming modes was significant when a cell swam near a surface. In contrast, the difference was indistinguishable when a cell swam >60 mu m away from any surfaces. In addition, a cell in backward mode tended to stay near the surface longer than a cell in forward mode. This wall effect on the bacterial motion was independent of chemical modi. cation of the glass surface. The macroscopic behavior is numerically simulated on the basis of experimental results and the significance of the phenomenon reported here is discussed.