The multitumor (sausage) tissue block: novel method for immunohistochemical antibody testing.
The multitumor (sausage) tissue block: novel method for immunohistochemical antibody testing.
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发表时间：
1986-08
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通讯作者：
H. Battifora
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作者：
H. Battifora
我们描述了一种将100个或更多不同组织样本包埋在正常大小的石蜡块中的方法，即多肿瘤组织块（MTTB）。MTTB允许用一滴抗体在单个载玻片上同时对许多组织样本进行免疫组织学检测。由于所有组织样本在免疫染色过程中均得到同等处理，因此消除了大多数变异来源，便于进行比较研究。含有各种肿瘤的MTTB适合于在单克隆抗体生产的早期阶段筛选杂交瘤上清液。这允许经济和快速筛选组织特异性单克隆抗体，并同时选择那些在组织切片中表现良好的。MTTB也可用于免疫组织化学中灵敏度的常规监测，因为它们可以制备成包括具有广谱抗原密度的组织。由于可以从单个MTTB获得大量几乎相同的切片，因此这些切片可用于大规模实验室间质量控制研究。
We describe a method of embedding 100 or more different tissue samples in a normal-sized paraffin block, the multitumor tissue block (MTTB). MTTBs allow the simultaneous immunohistologic testing of numerous tissue samples on a single slide with one drop of antibody. Because all of the tissue samples are treated equally during immunostaining, most sources of variation are removed and comparative studies are facilitated. MTTBs containing miscellaneous neoplasms are suitable for screening of hybridoma supernatants during the early phases of monoclonal antibody production. This permits economical and rapid screening for tissue-specific monoclonal antibodies and simultaneous selection of those that perform well in tissue sections. MTTBs are also useful for the routine monitoring of sensitivity in immunohistochemistry because they can be prepared to include tissues with a broad spectrum of antigen densities. Because a large number of nearly identical sections can be obtained from a single MTTB, these sections could be useful for large scale interlaboratory quality control studies.