Boron nutrition of cultured tobacco BY-2 cells. VII. Rapid induction of metabolic dysfunction in cells deprived of boron as revealed by microarray analysis
Boron nutrition of cultured tobacco BY-2 cells. VII. Rapid induction of metabolic dysfunction in cells deprived of boron as revealed by microarray analysis
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培养烟草 BY-2 细胞的硼营养。
DOI：
10.1080/00380768.2013.763383
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Matoh T
中科院分区：
文献类型：
--
作者：
Koshiba T.;Kobayashi M.;Matsuoka K.;Fujiwara T.;Matoh T
在植物中，硼（B）通过与果胶形成硼酸酯交联来构建细胞壁结构，并且这种元素的缺乏会导致氧化损伤和细胞死亡。然而，B缺乏导致这种灾难的过程还没有得到很好的理解。在这项研究中，我们研究了B剥夺培养烟草（Nicotiana tabacumL.）BY-2细胞的cDNA微阵列。结果表明，剥夺B的细胞上调其抗氧化防御系统，以科普应激条件。然而，适应性反应似乎在剥夺的第一个12小时内下降。缺硼12 h后，细胞四氮唑还原活性低于对照组。这些结果共同表明，在B-剥夺细胞的细胞功能严重受损，早在剥夺后12小时。B剥夺诱导的损伤的快速发作表明与B共价交联的果胶网络的重要性。
In plants, boron (B) works to build the cell wall structure by forming borate ester cross-links with pectin, and the deficiency of this element brings about oxidative damage and cell death. However, the process via which B deficiency leads to such catastrophe has not been well understood. In this study, we investigated the temporal changes in transcript profiles of B-deprived cultured tobacco (Nicotiana tabacumL.) BY-2 cells using cDNA microarray. The results suggest that the cells deprived of B up-regulated their antioxidative defense system to cope with the stress condition. However, the adaptive responses seemed to have declined within the first 12 h of deprivation. Tetrazolium reducing activity of B-deprived cells was lower than that of control cells at 12 h. These results together suggest that cellular function in B-deprived cells was seriously damaged as early as 12 h after deprivation. The rapidity of the onset of B deprivation-induced damages suggests the importance of the pectic network covalently cross-linked with B.