Phagocytosis is regulated by nitric oxide in murine microglia
Phagocytosis is regulated by nitric oxide in murine microglia
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DOI：
10.1006/niox.2000.0280
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发表时间：
2000-04-01
影响因子：
3.9
通讯作者：
Carroll, RT
中科院分区：
文献类型：
--
作者：
Kopec, KK;Carroll, RT
一氧化氮（NO）是由活化的小胶质细胞中的诱导型一氧化氮合酶（iNOS）产生的，并已被证明参与宿主防御机制。然而，由组成型一氧化氮合酶（cNOS）产生的NO在小胶质细胞中的作用尚不清楚。在本报告中，流式细胞术定量发现NO可调节小鼠BV-2小胶质细胞的吞噬功能，与未处理的小胶质细胞相比，添加NO生成化合物可导致吞噬功能受损，添加一氧化氮合酶（NOS）抑制剂可增强小胶质细胞的吞噬功能，提示组成型NO参与了吞噬功能的调节。当添加阿尔茨海默氏β -淀粉样蛋白肽时，NO的产生与吞噬也呈负相关。与β -淀粉样蛋白处理，组成性一氧化氮产生减少，而吞噬增加。从未处理的小胶质细胞中提取的细胞提取物中发现含有神经元和内皮细胞的NOS亚型，但不含诱导型。自发NO产生与减弱吞噬的相关性表明，组成型NOS酶参与了小胶质细胞的调节。(C) 2000年学术出版社。
Nitric oxide (NO) is produced by inducible nitric oxide synthase (iNOS) in activated microglia and has been shown to participate in host defense mechanisms. However, the role of NO produced by constitutive nitric oxide synthase (cNOS) in microglia is poorly understood. in this report, NO was found to regulate phagocytosis in murine BV-2 microglial cells as quantified by flow cytometry, Addition of NO-generating compounds caused impaired phagocytosis as compared to untreated microglia, The addition of nitric oxide synthase (NOS) inhibitors to microglial cells resulted in potentiation of phagocytosis, suggesting that constitutive NO was participating in the regulation of phagocytosis, The inverse correlation between NO production and phagocytosis was also observed when Alzheimer's beta-amyloid peptide was added. With beta-amyloid treatment, constitutive NO production decreased while phagocytosis increased. Cell extracts prepared from untreated microglia were found to contain both neuronal and endothelial NOS isoforms, but not the inducible form, The correlation of spontaneous NO production with attenuated phagocytosis suggests that constitutive NOS enzymes participate in microglial regulation. (C) 2000 Academic Press.