Transcriptional profiling of Caulobacter crescentus during growth on complex and minimal media
Transcriptional profiling of Caulobacter crescentus during growth on complex and minimal media
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DOI：
10.1128/jb.186.5.1448-1461.2004
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发表时间：
2004-03-01
影响因子：
3.2
通讯作者：
Stephens, C
中科院分区：
文献类型：
--
作者：
Hottes, AK;Meewan, M;Stephens, C
微阵列分析用于研究淡水贫营养细菌新月柄杆菌在三种标准实验室培养基（蛋白胨-酵母提取物培养基（PYE）和以葡萄糖或木糖为碳源的最小盐培养基）上生长期间的基因表达。近400个基因（约占基因组的10%）在这些培养基中的至少两种之间的表达显著不同。差异表达的基因包括许多编码运输系统，最显着的不同TonB依赖的外膜通道未知的底物特异性。氨基酸降解途径构成PYE中诱导的最大类基因。相反，在基本培养基中上调的许多基因编码合成氨基酸的酶，包括将氨和硫酸盐掺入谷氨酸和半胱氨酸。葡萄糖的可用性诱导表达的基因编码酶的Entner-Doudorolf途径，这是通过突变分析证明在这里是必不可少的C crescentus葡萄糖上的增长。木糖诱导编码几种水解外切酶的基因以及可能编码木糖催化酶的新途径的操纵子的表达。一个保守的DNA基序上游的许多木糖诱导的基因被确定，并显示赋予木糖特异性表达。木糖是植物细胞壁中木聚糖的丰富组分，并且微阵列数据表明，除了作为新月形木聚糖生长的碳源之外，这种戊糖可以被解释为产生与植物聚合物降解相关的酶的信号。
Microarray analysis was used to examine gene expression in the freshwater oligotrophic bacterium Caulobacter crescentus during growth on three standard laboratory media, including peptone-yeast extract medium (PYE) and minimal salts medium with glucose or xylose as the carbon source. Nearly 400 genes (approximately 10% of the genome) varied significantly in expression between at least two of these media. The differentially expressed genes included many encoding transport systems, most notably diverse TonB-dependent outer membrane channels of unknown substrate specificity. Amino acid degradation pathways constituted the largest class of genes induced in PYE. In contrast, many of the genes upregulated in minimal media encoded enzymes for synthesis of amino acids, including incorporation of ammonia and sulfate into glutamate and cysteine. Glucose availability induced expression of genes encoding enzymes of the Entner-Doudorolf pathway, which was demonstrated here through mutational analysis to be essential in C crescentus for growth on glucose. Xylose induced expression of genes encoding several hydrolytic exoenzymes as well as an operon that may encode a novel pathway for xylose catabolism. A conserved DNA motif upstream of many xylose-induced genes was identified and shown to confer xylose-specitic expression. Xylose is an abundant component of xylan in plant cell walls, and the microarray data suggest that in addition to serving as a carbon source for growth of C crescentus, this pentose may be interpreted as a signal to produce enzymes associated with plant polymer degradation.