Proteomic profiling in pancreatic cancer with and without lymph node metastasis
Proteomic profiling in pancreatic cancer with and without lymph node metastasis
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DOI：
10.1002/ijc.24163
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发表时间：
2009-04-01
影响因子：
6.4
通讯作者：
Han, Jinxiang
中科院分区：
文献类型：
--
作者：
Cui, Yazhou;Wu, Jianmei;Han, Jinxiang
本研究的目的是观察胰腺癌淋巴结转移（LNM）与非淋巴结转移（LNM）的差异蛋白表达谱，寻找新的LNM相关蛋白，为进一步了解LNM的转移机制和治疗提供靶点。从8例LNM和7例非LNM胰腺癌组织中手工显微切割癌巢，然后通过差异凝胶电泳（DIGE）分离蛋白质提取物，并通过MALDI-TOF-TOF鉴定。四个不同的调节蛋白，ezrin，radixin，moesin，和c14 orf 166，被选中进一步验证通过Western印迹和免疫组化。在DIGE分析中，我们确定了18个上调蛋白和15个下调蛋白在LNM胰腺癌巢相比，非LNM的。Western印迹和免疫组化分析证实，radixin，moesin和c14 orf 166，但不是ezrin，有显着较高的表达水平在LNM胰腺癌比非LNM对照。结论：本研究中发现的特异性蛋白质谱可能为研究淋巴结转移机制提供新的思路。c14 orf 166首次被鉴定为一种新的转移相关蛋白，radixin、moesin和c14 orf 166在肿瘤转移中的作用值得进一步研究。(C)2008 Wiley-Liss，Inc.
The aim of the study was to observe different protein profiles in pancreatic cancer with and without lymph node metastasis (LNM), and search for novel LNM-associated proteins, which would help to understand the metastatic mechanisms and provide targets for therapeutic interventions. Cancer nests were manually miscrodissected from 8 LNM and 7 non-LNM pancreatic cancer tissues, and the protein extracts were then separated by difference gel electrophoresis (DIGE) and identified by MALDI-TOF-TOF. Four differently regulated proteins, ezrin, radixin, moesin, and c14orf166, were selected for further validation by Western blot and immunohistochemistry. In DIGE analysis, we identified 18 up-regulated proteins and 15 down-regulated proteins in LNM pancreatic cancer nests compared with non-LNM ones. Western blot and immunohistochemical analyses confirmed that radixin, moesin and c14orf166, but not ezrin, had significantly higher expression levels in LNM pancreatic cancers than in non-LNM controls. In conclusion, the specific protein profiles found in this study might provide new insights into the mechanism of lymph node metastasis. For the first time, c14orf166 was identified as a novel metastasis-associated protein, and the roles of radixin, moesin and c14orf166 in cancer metastasis deserve further investigations. (C) 2008 Wiley-Liss, Inc.