Characterization of wheat-Thinopyrum partial amphiploids by meiotic analysis and genomic in situ hybridization
Characterization of wheat-Thinopyrum partial amphiploids by meiotic analysis and genomic in situ hybridization
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DOI：
10.1139/gen-43-4-712
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发表时间：
2000-08-01
期刊：
影响因子：
3.1
通讯作者：
Armstrong, KW
中科院分区：
文献类型：
--
作者：
Fedak, G;Chen, Q;Armstrong, KW
采用基因组原位杂交（GISH）和减数分裂配对分析相结合的方法，对小麦-偃麦草部分双倍体的基因组组成和亲缘关系进行了研究。根据减数分裂行为和GISH图谱的相似性，初步判定TAF 46和Otrastayuskaya 38这两个部分双倍体的外源染色体来源于Th。intermedium，而Th. ponticum是其他6个部分双二倍体：PWM 706、PWM 206、PWM 209、PWMIII、OK 7211542和Ag-小麦杂种的亲本之一。发现这些部分双倍体中的每一个都含有由St-、J-或J（s）-基因组染色体的不同组合组成的合成的外来基因组。对于部分双倍体系的亲缘关系，F-1杂种的减数分裂分析和GISH结果通常是互补的，但后者在鉴定组成基因组方面提供了更高的精度。
A combination of genomic in situ hybridization (GISH) and meiotic pairing analysis of wheat-Thinopyrum partial amphiploids was employed to identify the genomic constitution and relationships between partial amphiploids derived from wheat and wheatgrass crosses. On the basis of similarities in the meiotic behavior and GISH patterns, the alien chromosomes of two of eight partial amphiploids, TAF46 and 'Otrastayuskaya 38', were judged to originate from Th. intermedium, whereas Th. ponticum was one of the parents of the other six partial amphiploids; PWM706, PWM206, PWM209, PWMIII, OK7211542, and Ag-wheat hybrid. Each of these partial amphiploids was found to contain a synthetic alien genome composed of different combinations of St-, J-, or J(s)-genome chromosomes. For relatedness of partial amphiploid lines, meiotic analysis of F-1 hybrids and GISH results were generally complementary, but the latter offered greater precision in identifying constituent genomes.