Regulation of T-cell-independent and T-cell-dependent antibody production by circadian rhythm and melatonin
Regulation of T-cell-independent and T-cell-dependent antibody production by circadian rhythm and melatonin
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DOI：
10.1093/intimm/dxp109
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发表时间：
2010-01-01
影响因子：
4.4
通讯作者：
Girkontaite, Irute
中科院分区：
文献类型：
--
作者：
Cernysiov, Vitalij;Gerasimcik, Natalija;Girkontaite, Irute
褪黑素是一种具有免疫调节活性的激素，据信会影响哺乳动物抗体的产生。本研究的目的是探讨抑制褪黑素合成对抗体产生的影响。用t细胞依赖性（TD）和t细胞非依赖性（TI）抗原免疫BALB/c小鼠，并将其置于(i)正常光照、（ii）持续光照、（iii）光照和每日褪黑素处理下。结果表明，褪黑激素调节了TD和TI抗体的产生。抑制褪黑素合成后，TI抗原免疫后IgM、IgG1、IgG2b和IgG3抗体的数量增加。光照小鼠的TD抗体IgM、IgG2a、IgG2b和IgG3水平均升高，但IgG1同型抗原特异性抗体水平明显降低。每日褪黑素治疗使抗体水平恢复正常。夜间免疫时，正常光照下小鼠血清中抗体浓度明显升高。褪黑素通过MT2受体对B细胞的作用在体内和体外均得到了证实。
Melatonin is a hormone that has immunomodulatory activity and is believed to influence the production of antibodies in mammals. The aim of the present study was to investigate the effect of suppressed melatonin synthesis on the antibody production. BALB/c mice were immunized with T-cell-dependent (TD) and T-cell-independent (TI) antigens and kept under (i) normal lighting, (ii) constant exposure to light, (iii) exposed to light and treated daily with melatonin. It was revealed that melatonin modulated TD and TI antibody production. Suppressed melatonin synthesis increased the amount of IgM, IgG1, IgG2b and IgG3 antibodies after immunization with TI antigen. The level of TD antibodies IgM, IgG2a, IgG2b and IgG3 also increased, however, the antigen-specific antibodies of IgG1 isotype significantly decreased in mice exposed to light. Daily melatonin treatment brought the antibody level back to normal. The antibody concentration in the sera of mice kept at normal lighting was significantly higher when the immunizations were performed in the evening. The action of melatonin on B cells via MT2 receptor was shown in vitro and in vivo.