Genetics of resistance to phosphonoacetic acid in strain KOS of herpes simplex virus type 1
Genetics of resistance to phosphonoacetic acid in strain KOS of herpes simplex virus type 1
复制标题
1型单纯疱疹病毒KOS株对膦酰乙酸的抗性遗传学
作者：
J. Jofre;P. Schaffer;D. Parris
表现出不耐热 DNA 聚合酶活性的 1 型单纯疱疹病毒 DNA 温度敏感突变体 tsD9，在允许的温度下铺板时显示出对膦酰乙酸 (PAA) 具有抗性。 tsD9 的 ts+ 回复体对 PAA 敏感，并且表现出介于野生型病毒和 tsD9 之间的 DNA 聚合酶活性，表明温度敏感性和对 PAA 的敏感性均由同一基因控制。由于 tsD9 在现有单纯疱疹病毒 1 型连锁图上的位置已知，因此 PAA 抗性基因座（以及病毒 DNA 聚合酶的结构基因）已被识别。
A DNA- temperature-sensitive mutant of herpes simplex virus type 1 exhibiting thermolabile DNA polymerase activity, tsD9, was shown to be resistant to phosphonoacetic acid (PAA) when plated at the permissive temperature. ts+ revertants of tsD9 were PAA sensitive and exhibited DNA polymerase activity intermediate between that of the wild-type virus and tsD9, indicating that both temperature sensitivity and sensitivity to PAA are controlled by the same gene. Since the position of tsD9 on the existing herpes simplex virus type 1 linkage map is known, the locus for PAA resistance--and therefore for the structural gene for viral DNA polymerase--has been identified.