Effect of temperature on sulphate reduction, growth rate and growth yield in five psychrophilic sulphate-reducing bacteria from Arctic sediments
Effect of temperature on sulphate reduction, growth rate and growth yield in five psychrophilic sulphate-reducing bacteria from Arctic sediments
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DOI：
10.1046/j.1462-2920.1999.00061.x
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发表时间：
1999-10-01
影响因子：
5.1
通讯作者：
Jorgensen, BB
中科院分区：
文献类型：
--
作者：
Knoblauch, C;Jorgensen, BB
五株嗜冷硫酸盐还原菌对从北极沉积物中分离到的5株（ASv 26、LSv 21、PSv 29、LSv 54和LSv 514）进行了长期低温适应性研究。所有菌株均能在海水冰点-1.8 ℃下生长，但最适生长温度（T-opt）分别为7 ℃（PSv 29）、10 ℃（PSv 29）和10 ℃（PSv 514）。（ASv 26，LSv 54）和18 ℃（LSv 21，LSv 514），虽然T-opt明显高于其栖息地的原位温度（-1.7摄氏度和2.6摄氏度），0摄氏度时相对增长率仍然很高，短期培养的指数增长的文化表明，最高的硫酸盐还原率发生2-9摄氏度以上的T-与生长和硫酸盐还原速率相反，菌株ASv 26、LSv 54和PSv 29的生长产量在-1.8 ℃和T-opt之间几乎恒定。然而，对于菌株LSv 21和LSv 514，生长产量在最低温度（约0 ℃）下最高。结果表明，嗜冷硫酸盐还原菌特别适合于永久低温高相对生长速率和高生长产量在现场条件下。
Five psychrophilic sulphate-reducing bacteria (strains ASv26, LSv21, PSv29, LSv54 and LSv514) isolated from Arctic sediments were examined for their adaptation to permanently low temperatures, All strains grew at -1.8 degrees C, the freezing point of sea water, but their optimum temperature for growth (T-opt) were 7 degrees C (PSv29), 10 degrees C (ASv26, LSv54) and 18 degrees C (LSv21, LSv514), Although T-opt was considerably above the in situ temperatures of their habitats (-1.7 degrees C and 2.6 degrees C), relative growth rates were still high at 0 degrees C, accounting for 25-41% of those at T-opt. Short-term incubations of exponentially growing cultures showed that the highest sulphate reduction rates occurred 2-9 degrees C above T-opt. In contrast to growth and sulphate reduction rates, growth yields of strains ASv26, LSv54 and PSv29 were almost constant between -1.8 degrees C and T-opt. For strains LSv21 and LSv514, however, growth yields were highest at the lowest temperatures, around 0 degrees C. The results indicate that psychrophilic sulphate-reducing bacteria are specially adapted to permanently low temperatures by high relative growth rates and high growth yields at in site conditions.