IMMUNOHISTOCHEMICAL LOCALIZATION OF HUMAN-LIVER GLUTATHIONE S-TRANSFERASE (GST) ISOZYMES WITH SPECIAL REFERENCE TO POLYMORPHIC GST1
IMMUNOHISTOCHEMICAL LOCALIZATION OF HUMAN-LIVER GLUTATHIONE S-TRANSFERASE (GST) ISOZYMES WITH SPECIAL REFERENCE TO POLYMORPHIC GST1
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DOI：
10.1016/0167-4838(89)90047-2
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发表时间：
1989-05-01
期刊：
影响因子：
--
通讯作者：
OSUGA, T
中科院分区：
文献类型：
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作者：
ABEI, M;HARADA, S;OSUGA, T
对3个人谷胱甘肽S转移酶(RX：谷胱甘肽R转移酶，EC2.5.1.18)(GST)(GST-1、GST-2和GST-3)的产物进行了纯化，并对其在肝脏中的免疫组织化学定位进行了研究，重点研究了中性同工酶GST-1的多态性。GST1均匀分布于肝小叶细胞胞浆中，表现为GST1-1、GST1-2和GST1-2-1三种表型。而表现GST10表型的肝组织无一例染色。免疫组织化学GST2(碱性同工酶)染色均匀分布于肝细胞胞浆中，分布于肝小叶，胞核也呈强阳性染色。GST3(酸性同工酶)在胆管上皮细胞表达较强，而肝细胞表达不明显。这些结果表明，人肝GST同工酶在个体间、细胞内和细胞器内的分布存在显著差异。
The products of three human glutathione S-transferase (RX:glutathione R-transferase, EC 2.5.1.18) (GST) loci (GST 1, GST 2 and GST 3) were purified and their immunohistochemical localization in liver was studied with special attention to the polymorphism of GST1 (neutral isozyme). The GST1 was homogeneously stained in cytoplasm of hepatocytes throughout the lobule of liver showing GST1 1, GST1 2 and GST1 2-1 phenotypes. However, none of the hepatic tissue showing GST1 0 phenotype was stained. Immunohistochemical staining of GST2 (basic isozyme) was distributed in the cytoplasm of hepatocytes homogeneously throughout the hepatic lobule in all cases and the strong staining intensity was also demonstrated in nucleus. GST3 (acidic isozyme) was strongly stained in biliary epithelium, while staining of hepatocytes was not apparent. These results indicate that the human liver GST isozymes exhibit significant difference in their inter-individual, specific cellular and organellar distribution.