A combined analysis of transcriptomics and proteomics of a novel Antarctic Salpa sp. and its potential toxin screenings
A combined analysis of transcriptomics and proteomics of a novel Antarctic Salpa sp. and its potential toxin screenings
复制标题
新型南极 Salpa sp 的转录组学和蛋白质组学的综合分析。
DOI：
10.1016/j.ijbiomac.2020.05.144
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发表时间：
2020
影响因子：
8.2
通讯作者：
Xiao Liang
中科院分区：
文献类型：
--
作者：
Ye Ruiwei;Liang Hongyu;Chen Ting;Zhang Jie;Yang Fengling;Chen Xintong;Li Xiaoya;Gao Songyu;Yang Ying;Wang Yongqiang;Tao Feng;Zhang Jing;Xiao Liang
BackgroundWe successfully captured a kind of gelatinous organism DA-6 from Antarctic water, extracted its total RNAs and proteins, and performed species identification through a combination of transcriptomics and proteomics in this study.MethodsThe gelatinous organism DA-6 was captured 200 m underwater in Antarctica. Total RNA was extracted to construct the transcriptome and the proteins were identified by LC-MS/MS.ResultsDA-6 was identified as an AntarcticSalpasp. through morphological examination and MT-CO1 phylogenetic analysis. A total of 47,183 unigenes were harvested through transcriptome. We also successfully annotated 11,954 (25.34%), 10,006 (22.21%), 4469 (9.47%) and 4901 (9.71%) unigenes with NR, SwissProt, GO and KEGG databases, respectively. In the proteomic analysis, a total of 4680 peptides and 1280 proteins were harvested using the transcriptome as the reference database. A number of both 549 (31.98%) proteins reannotated against the GO and KEGG databases. Moreover, a number of 5 toxic proteins matched from the 89 toxin-related unigenes were successfully screened, including 2 metalloproteinases, 1 serine protease, 1 serine protease inhibitor and 1 aflatoxin.ConclusionOur study is the first to identify an AntarcticSalpasp. according to the combination of de novo transcriptomics and proteomics, which can further be served as a public database for the identification of potential polarSalpa-derived lead compounds. In addition to morphology and CO1, the combined analysis of transcriptome and proteome can also be used as a value method for new species identify e.g.Salpasp.
