Involvement of PKA signalling in anti‐inflammatory effects of chitosan oligosaccharides in IPEC‐J2 porcine epithelial cells
Involvement of PKA signalling in anti‐inflammatory effects of chitosan oligosaccharides in IPEC‐J2 porcine epithelial cells
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DOI：
10.1111/jpn.12686
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发表时间：
2018-02
影响因子：
2.7
通讯作者：
J. W. Yang;G. Tian;D. Chen;Y. Yao;J. He;P. Zheng;X. Mao;J. Yu;Z. Huang;B. Yu
中科院分区：
文献类型：
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作者：
J. W. Yang;G. Tian;D. Chen;Y. Yao;J. He;P. Zheng;X. Mao;J. Yu;Z. Huang;B. Yu
断奶以肠道炎症为特征，是养猪业面临的一大挑战。控制肠道炎症对于改善生长性能和健康是重要的。因此，研究了低分子量壳寡糖（LCOS）在猪小肠上皮细胞系（IPEC-J2）中的抗炎活性。结果表明，TNF-α作为炎症诱导剂，可显著上调IL-8和MCP-1 mRNA的表达。LPS可明显抑制TNF-α诱导的细胞内IL-8和MCP-1 mRNA的表达。甘露糖受体的配体甘露糖（Mannose，MAN）对LPS的抗炎活性无影响，提示甘露糖受体可能不参与LPS对IPEC-J2细胞的抗炎作用。有趣的是，N-[2-（p-bromocinylamino）ethyl]-5-isoquinolinesulfonamide 2 HCl hydrate（H89）作为PKA（protein kinase A）特异性抑制剂，在与LCOS共培养时逆转IL-8的mRNA表达。与TNF-α处理组相比，LPS浓度依赖性下调claudin-1 mRNA的表达。LPS与TNF-α共培养3 h后，对小肠跨上皮电阻（TEER）无明显影响。因此，LPS具有较强的抗炎活性，可作为饲料添加剂用于抑制断奶仔猪肠道炎症的发生。
Weaning is characterized by intestinal inflammation, which is a big challenge in pig industry. Control of intestinal inflammation is important for improvement of growth performance and health. Therefore, the study was focused on the anti-inflammatory activity of low-molecular-weight chitosan oligosaccharide (LCOS) in a porcine small intestinal epithelial cell line (IPEC-J2). The results showed that TNF-α, as inflammation inducer, significantly upregulated the mRNA expression of IL-8 and MCP-1. Afterwards, LCOS significantly attenuated mRNA expression of IL-8 and MCP-1 induced by TNF-α in the cells. Mannose (MAN), as ligand of mannose receptor, had no effect on the anti-inflammatory activity of LCOS, which suggested that mannose receptor may not involve in the anti-inflammatory activity of LCOS in IPEC-J2 cells. Interestingly, N-[2-(p-bromocinnamylamino)ethyl]-5-isoquinolinesulfonamide 2HCl hydrate (H89), as PKA (protein kinase A)-specific inhibitor, reversed the mRNA expression of IL-8 when co-cultured with LCOS. Furthermore, LCOS concentration dependent downregulated the mRNA expression of claudin-1 compared with TNF-α treatment. However, the trans-epithelial electric resistance (TEER) was not affected by LCOS when co-cultured with TNF-α in 3 hr. In conclusion, LCOS have a potent anti-inflammatory activity, and as a feed additives, may be useful for the inhibition of inflammatory process in weaning period of pigs with intestinal inflammation occurring.