Synergistic action of nectins and cadherins generates the mosaic cellular pattern of the olfactory epithelium.
Synergistic action of nectins and cadherins generates the mosaic cellular pattern of the olfactory epithelium.
复制标题
DOI：
10.1083/jcb.201509020
复制
发表时间：
2016-02-29
期刊：
影响因子：
--
通讯作者：
Togashi H
中科院分区：
文献类型：
--
作者：
Katsunuma S;Honda H;Shinoda T;Ishimoto Y;Miyata T;Kiyonari H;Abe T;Nibu K;Takai Y;Togashi H
嗅觉细胞和支持细胞之间的细胞重排，受不同表达和分布的胶凝素和钙粘附素的驱动，是嗅上皮中镶嵌细胞模式所必需的。在嗅觉上皮(OE)中，表达不同钙粘附素的嗅细胞(OCs)和支持细胞(SCs)呈典型的镶嵌模式排列，其中OCs被SCs包围。然而，这种细胞模式背后的机制尚不清楚。在这里，我们表明OE的细胞模式是通过发育过程中的细胞重排建立的。在OE中，OCs表达Nectin-2和N-cadherin，SC表达Nectin-2、Nectin-3、E-cadherin和N-cadherin。OCS上的Nectin-2和SCs上的Nectin-3之间的异嗜性反式相互作用优先通过α-连接素将钙粘素招募到异型连接，连接之间钙粘附素的差异分布促进了细胞内嵌，导致了镶嵌图案的形成。这些观察结果得到了模型细胞系统的证实，各种细胞模式是由蜜腺蛋白和钙粘附素的组合表达产生的。总体而言，胶凝素和钙粘附素的协同作用产生了马赛克图案，这不是单一机制所能实现的。
Cellular rearrangements between olfactory cells and supporting cells, driven by the different expression and distribution of nectins and cadherins, are required for mosaic cellular patterning in the olfactory epithelium. In the olfactory epithelium (OE), olfactory cells (OCs) and supporting cells (SCs), which express different cadherins, are arranged in a characteristic mosaic pattern in which OCs are enclosed by SCs. However, the mechanism underlying this cellular patterning is unclear. Here, we show that the cellular pattern of the OE is established by cellular rearrangements during development. In the OE, OCs express nectin-2 and N-cadherin, and SCs express nectin-2, nectin-3, E-cadherin, and N-cadherin. Heterophilic trans-interaction between nectin-2 on OCs and nectin-3 on SCs preferentially recruits cadherin via α-catenin to heterotypic junctions, and the differential distributions of cadherins between junctions promote cellular intercalations, resulting in the formation of the mosaic pattern. These observations are confirmed by model cell systems, and various cellular patterns are generated by the combinatorial expression of nectins and cadherins. Collectively, the synergistic action of nectins and cadherins generates mosaic pattern, which cannot be achieved by a single mechanism.