Characterization and immortalization of woodchuck hepatocytes isolated from normal and hepadnavirus-infected woodchucks (Marmota monax).
Characterization and immortalization of woodchuck hepatocytes isolated from normal and hepadnavirus-infected woodchucks (Marmota monax).
复制标题
从正常和感染嗜肝DNA病毒的土拨鼠（Marmota monax）中分离出的土拨鼠肝细胞的表征和永生化。
DOI：
10.1006/excr.1994.1116
复制
发表时间：
1994
影响因子：
3.7
通讯作者：
Tennant,BC
中科院分区：
文献类型：
--
作者：
Jacob,JR;Liu,RH;Roneker,CA;deNoronha,F;Hotchkiss,JH;Tennant,BC
在常规含血清培养基或无血清培养基中培养正常土拨鼠和慢性土拨鼠肝炎病毒（WHV）感染土拨鼠的原代土拨鼠（旱獭）肝细胞。血浆蛋白白蛋白、转铁蛋白、纤维蛋白原和补体C3的新生合成在两种条件下是相同的。然而，在无血清条件下，WHV的表达和一氧化氮的合成减少。原代土拨鼠肝细胞培养在传统的，含血清的培养基中永生化利用猿猴病毒40 T抗原癌基因。永生化肝细胞系保留了一氧化氮合成和补体C3表达的分化功能。土拨鼠肝细胞培养模型将补充目前的实验方法，允许调查嗜肝DNA病毒的发病机制，包括体外肝癌。
Primary woodchuck (Marmota monax) hepatocytes from normal woodchucks and woodchucks with chronic woodchuck hepatitis virus (WHV) infection were cultured in either a conventional serum-containing medium or a serum-free medium. Thede novosynthesis of the plasma proteins albumin, transferrin, fibrinogen, and complement C3 were identical under both conditions. However, expression of the WHV and the synthesis of nitric oxide were diminished under serum-free conditions. Primary woodchuck hepatocytes cultured in conventional, serum-containing medium were immortalized utilizing the simian virus 40 T antigen oncogene. Immortalized hepatic cell lines retained differentiated functions of nitric oxide synthesis and expression of complement C3. The woodchuck hepatocyte culture model will supplement current experimental methods, allowing investigation of hepadnaviral pathogenesis, including hepatocarcinogenesisin vitro.