EPITHELIAL WOUND-HEALING ENHANCED BY TRANSFORMING GROWTH FACTOR-ALPHA AND VACCINIA GROWTH-FACTOR
EPITHELIAL WOUND-HEALING ENHANCED BY TRANSFORMING GROWTH FACTOR-ALPHA AND VACCINIA GROWTH-FACTOR
复制标题
DOI：
10.1126/science.3492044
复制
发表时间：
1987-01-16
期刊：
影响因子：
56.9
通讯作者：
TODARO, GJ
中科院分区：
文献类型：
--
作者：
SCHULTZ, GS;WHITE, M;TODARO, GJ
皮肤中真皮损伤后的表皮再生需要角质形成细胞的增殖和迁移。表皮生长因子（EGF）在培养中刺激角质形成细胞的增殖，局部给药EGF可加速体内部分厚度烧伤或裂厚切口的表皮再生。转化生长因子- α (TGF-. α)和痘苗生长因子（VGF）与EGF具有相当的序列同源性，它们似乎都结合同一受体蛋白。是否TGF -.alpha。VGF在体内是否影响表皮伤口愈合尚不清楚。目前的研究表明，局部给药TGF-。或抗生素乳膏中的VGF对部分烧伤（二度）加速表皮再生与未治疗或车辆治疗的烧伤相比。低水平的TGF- α。而VGF（0.1微克/毫升）在刺激表皮再生方面似乎比EGF更有效。TGF- α治疗烧伤再生上皮。VGF在组织学上表现正常。这一发现表明，局部应用选定的生长因子可能有助于加速部分厚度损伤的愈合。
Epidermal regeneration following middermal injuries to skin requires both proliferation and migration of keratinocytes. Epidermal growth factor (EGF) stimulates the proliferation of keratinocytes in culture, and topical administration of EGF accelerates epidermal regeneration of partial thickness burns or split-thickness incisions in vivo. Transforming growth factor-alpha (TGF-.alpha.) and vaccinia growth factor (VGF) have substantial sequence homology with EGF, and all appear to bind to the same receptor protein. Whether TGF-.alpha. or VGF can affect epidermal wound healing in vivo is not known. The present studies show that topical administration of TGF-.alpha. or VGF in antibiotic cream to partial thickness burns (second degree) accelerated epidermal regeneration in comparison with untreated or vehicle-treated burns. Low levels of both TGF-.alpha. and VGF (0.1 microgram per milliliter) appeared to be more effective than EGF in stimulating epidermal regeneration. Regenerated epithelium from burns treated with TGF-.alpha. or VGF appeared normal histologically. This finding suggests that topical application of selected growth factors may be useful in accelerating healing of partial thickness injuries.