Evaluation of Brucella MLVA typing for human brucellosis
Evaluation of Brucella MLVA typing for human brucellosis
复制标题
DOI：
10.1016/j.mimet.2006.12.015
复制
发表时间：
2007-04-01
影响因子：
2.2
通讯作者：
Neubauer, Heinrich
中科院分区：
文献类型：
--
作者：
Al Dahouk, Sascha;Le Fleche, Philippe;Neubauer, Heinrich
人类布鲁氏菌病仍然是世界上最常见的细菌性人畜共患病。众所周知的分子工具和经典的生物分型方法都不能令人满意地对布鲁氏菌进行分型。尽管布鲁氏菌属具有高度的遗传同质性（DNA-DNA同源性bbb90 %），但含有可变数目串联重复序列（VNTRs）的位点最近证明了它们对动物源菌株分型的有效性。本研究的目的是评价MLVA（多位点VNTR分析）在人类布鲁氏菌病诊断和流行病学中的应用。利用8个小卫星标记（图1）和8个微卫星标记（图2）对所有3个生物变体的128株melitensis分离株进行了分型。共鉴定出110种不同的基因型。MLVA聚类模式与菌株的地理起源相关。从同一次暴发中的不同患者或从同一患者在一线治疗前和复发后分离的布鲁氏菌菌株显示相同的基因型。在密切相关的群体中发现了对紫红敏感的melitensis菌株，这表明VNTRs与某些表型特征之间存在关联。然而，经典微生物学方法建立的生物制剂的有效性无法通过MLVA聚类来证实。原始数据可在基因分型网页http://bacterial-genotyping上查询。igmors.u-psud.fr。MLVA测定在人布鲁氏菌分离株中具有快速、高度歧视性和可重复性。MLVA可显著促进布鲁氏菌感染的流行病学追溯分析，并可能促进人类布鲁氏菌病的监测和控制。(c) 2007 Elsevier B.V.版权所有
Human brucellosis is still the most common bacterial zoonosis worldwide. Neither well-known molecular tools nor the classical biotyping methods are satisfactory for subtyping of Brucella spp. Loci containing Variable Number of Tandem Repeats (VNTRs) have recently proved their usefulness in typing strains from animal origin despite the high genetic homogeneity within the genus Brucella (DNA-DNA homology > 90%).The aim of this study was to evaluate MLVA (Multiple Locus VNTR Analysis) for diagnostic and epidemiological use in human brucellosis. One hundred and twenty-eight B. melitensis isolates of all three biovars were typed using eight minisatellite (panel 1) and eight microsatellite (panel 2) markers. One hundred and ten different genotypes were identified. The MLVA clustering pattern correlated with the geographic origin of the strains. Brucella strains isolated from different patients within the same outbreak or from the same patient before first-line therapy and after relapse showed identical genotypes. Fuchsin sensitive B. melitensis strains were found in closely related clusters giving evidence for an association between VNTRs and some phenotypic characteristics. However, the validity of biovars established by classical microbiological methods could not be confirmed by MLVA clustering. The original data can be queried on the genotyping web page at http://bacterial-genotyping. igmors.u-psud.fr. The MLVA assay is rapid, highly discriminatory, and reproducible within human Brucella isolates. MLVA can significantly contribute to epidemiological trace-back analysis of Brucella infections and may advance surveillance and control of human brucellosis. (c) 2007 Elsevier B.V. All rights reserved.