The effect of blood coagulation factor XIII on fibrin clot structure and fibrinolysis
The effect of blood coagulation factor XIII on fibrin clot structure and fibrinolysis
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DOI：
10.1111/jth.12455
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发表时间：
2014-02-01
影响因子：
10.4
通讯作者：
Philippou, H.
中科院分区：
文献类型：
--
作者：
Hethershaw, E. L.;La Corte, A. L. Cilia;Philippou, H.
研究背景凝血因子XIII是一种分子量为320 kDa的四聚体，由两个酶促A亚基和两个载体B亚基组成（FXIII A（2）B（2））。活化的FXIII（FXIIIa）催化β-葡聚糖的形成。（-谷氨酰）赖氨酰共价键之间的-，-和-链相邻的纤维蛋白分子，也交联的主要纤溶酶抑制剂，2-抗纤溶酶，以fibrin.ObjectivesWe研究的作用FXIII交联的纤维蛋白直接在凝块形态和其功能的影响凝块形成和溶解，结果和结论我们的数据显示在凝块形成过程中FXIII的存在导致纤维蛋白凝块的孔径显著减小2.1倍，如通过达西常数Ks所确定的，与未加FXIII组（86.0 ± 1.7nm，P <0.01）相比，FXIII组纤维更细（74.7 ± 1.5nm，P <0.01），纤维密度更高（86.0 ± 1.7nm，P
BackgroundFactor XIII is a 320kDa tetramer, comprising two enzymatic A-subunits and two carrier B-subunits (FXIII A(2)B(2)). Activated FXIII (FXIIIa) catalyses the formation of epsilon-(-glutamyl)lysyl covalent bonds between -, - and - chains of adjacent fibrin molecules and also cross-links the major plasmin inhibitor, 2-antiplasmin, to fibrin.ObjectivesWe investigated the role of FXIII cross-linking of fibrin directly in clot morphology and its functional effect on clot formation and lysis, in the absence of 2-antiplasmin.Results and ConclusionsOur data show that the presence of FXIII during clot formation results in fibrin clots that have a significant 2.1-fold reduction in pore size, as determined by the Darcy constant, Ks, and formed thinner fibers (74.7 +/- 1.5nm) and higher density of fibers compared with those without FXIII (86.0 +/- 1.7nm, P