Genetics of somatic mammalian cells. IX. Quantitation of mutagenesis by physical and chemical agents
Genetics of somatic mammalian cells. IX. Quantitation of mutagenesis by physical and chemical agents
复制标题
哺乳动物体细胞的遗传学。
DOI：
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发表时间：
1969
影响因子：
5.6
通讯作者：
T. Puck
中科院分区：
文献类型：
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作者：
F. Kao;T. Puck
先前描述的在中国仓鼠细胞中诱导单基因突变的系统已经扩展到产生额外的营养缺陷突变体。本文提出了一种改进的方法来定量测定单基因突变对特定营养缺陷的效率。在用甲磺酸乙酯、N -甲基- N1 -硝基- N -亚硝基胍、羟胺、吖啶芥（ICR - 191）、咖啡因和紫外线和X射线照射处理这些细胞后，测量了正向诱变。测定各药剂单细胞存活曲线及染色单体断裂和重排效率。对水溶性致癌物N -亚硝基somethylurea也进行了类似的测量，结果表明该致癌物在产生营养不良的体细胞突变方面是有效的。这些结果为阐明细胞杀伤、染色体畸变、单基因突变和各种药物产生的癌变之间的关系提供了希望。所述方法可用于药物、食品添加剂和环境污染物在体外对哺乳动物细胞的诱变作用的常规检测。
The system previously described for inducing single gene mutations in Chinese hamster cells has been extended to produce additional auxotrophic mutants. An improved method for quantitating the efficiency of single gene mutation to specific auxotrophies has been developed. Mutagenesis in the forward direction has been measured after treatment of these cells with ethyl methanesulfonate, N‐methyl‐N1‐nitro‐N‐nitrosoguanidine, hydroxylamine, an acridine mustard (ICR‐191), caffeine and ultraviolet‐ and X‐irradiation. For each agent, the single cell survival curve and the efficiency of chromatid breakage and rearrangement were measured. Similar measurements were also carried out with a water‐soluble carcinogen N‐nitrosomethylurea, which was shown to be effective in producing auxotrophic, somatic mutations. These results offer promise of illuminating the relationships between cell killing, chromosomal aberration, single gene mutations and carcinogenesis produced by various agents. The methods described can be used in routine testing of drugs, food additives, and environmental pollutants for mutagenic action in mammalian cells in vitro.