Patterned plasmonic gradient for high-precision biosensing using a smartphone reader
Patterned plasmonic gradient for high-precision biosensing using a smartphone reader
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使用智能手机读取器进行高精度生物传感的图案等离子体梯度
DOI：
10.1039/c9nr00455f
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发表时间：
2019-07-14
期刊：
影响因子：
6.7
通讯作者：
Zhang, Weihua
中科院分区：
文献类型：
--
作者：
Bian, Jie;Xing, Xing;Zhang, Weihua
与智能手机兼容的生物传感器被认为是提高偏远地区诊断质量的关键技术之一。然而，迄今为止，由于手机携带的消费级探测器的局限性，很少有与智能手机兼容的生物传感器能够达到传统同类生物传感器的规格。为了解决这个问题，我们报道了一种受超表面启发的生物传感器，即图案化等离子体梯度（PPG），它将局部索引信息转换为二维图案。通过利用现代智能手机强大的成像和计算能力，PPG 足够灵敏，能够在大动态范围内高精度 (Delta n < 0.001) 检测由亚单层分子引起的微小折射率变化。它使我们能够实时监测生物素和微量链霉亲和素（15 nM，20 μL）之间的结合过程。 PPG具有高灵敏度和高精度，为专业设备无法到达的地方提供高性能的生物传感解决方案。
Smartphone-compatible biosensors are believed to be one of the key techniques for improving the quality of diagnosis in remote areas. However, to date, few smartphone-compatible biosensors can reach the specifications of their conventional counterparts due to the limitations of consumer-grade detectors carried by phones. To circumvent this issue, we reported a metasurface-inspired bio-sensor, patterned plasmonic gradient (PPG), which transduces local index information into 2D patterns. By harnessing the powerful imaging and computational capability of modern smartphones, the PPG is sensitive enough to detect tiny refractive index changes induced by a submonolayer of molecules with high precision (Delta n < 0.001) in a large dynamic range. It allows us to monitor the conjugation process between biotin and a trace amount of streptavidin (15 nM, 20 mu L) in real-time. With high sensitivity and accuracy, the PPG provides a high performance bio-sensing solution for the places where professional equipment is inaccessible.