Lactobacillus rhamnosus GR-1 Alleviates Escherichia coli-Induced Inflammation via NF-κB and MAPKs Signaling in Bovine Endometrial Epithelial Cells.
Lactobacillus rhamnosus GR-1 Alleviates Escherichia coli-Induced Inflammation via NF-κB and MAPKs Signaling in Bovine Endometrial Epithelial Cells.
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DOI：
10.3389/fcimb.2022.809674
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发表时间：
2022
影响因子：
5.7
通讯作者：
Liu M
中科院分区：
文献类型：
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作者：
Liu J;Feng X;Li B;Sun Y;Jin T;Feng M;Ni Y;Liu M
大肠埃希菌是奶牛子宫内膜炎的主要致病菌，严重降低了奶牛养殖业的经济效益。据报道，益生菌的消费对免疫调节有有益的影响。然而，益生菌对奶牛子宫内膜炎的炎症调控机制尚不清楚。本工作旨在阐明鼠李糖乳杆菌GR-1(L.rhamnosus GR-1)对抗大肠杆菌诱导的牛子宫内膜上皮细胞(BEECs)炎症损伤的机制。以健康奶牛子宫为材料，利用大肠杆菌建立BEECs细胞炎性损伤模型。在大肠杆菌诱导的子宫内膜炎的BEECs中，研究了添加鼠李糖GR-1对炎症的影响。进一步探讨了鼠李糖GR-1在大肠杆菌刺激的BEEC中的抗炎作用机制。结果表明，单用鼠李糖GR-1不能引起炎症因子的变化，而鼠李糖GR-1能显著减轻大肠杆菌诱导的炎症因子的表达。进一步的研究表明，鼠李糖GR-1显著抑制了大肠杆菌刺激的TLR4和MyD88的表达。此外，我们观察到在BEEC中，鼠李糖GR-1可以抑制大肠杆菌诱导的与NF-κB和MAPK相关的途径蛋白的表达。总之，鼠李糖GR-1对大肠杆菌诱导的炎症反应具有有效的保护作用，其机制可能与抑制TLR4和MyD88刺激NF-κB和MAPK有关。
Escherichia coli counts as a major endometritis-causing pathogen among dairy cows, which lowered the economic benefits of dairy farming seriously. Probiotic consumption has been reported to impart beneficial effects on immunomodulation. However, the inflammatory regulation mechanism of probiotics on endometritis in dairy cows remains unexplored. The current work aimed to clarify the mechanism whereby Lactobacillus rhamnosus GR-1 (L. rhamnosus GR-1) resists bovine endometrial epithelial cells (BEECs) inflammatory injury induced by E. coli. The model of cellular inflammatory injury was established in the BEECs, which comes from the uterus of healthy dairy cows using E. coli. The outcome of L. rhamnosus GR-1 addition on inflammation was evaluated in BEECs with E. coli-induced endometritis. The underlying mechanisms of anti-inflammation by L. rhamnosus GR-1 were further explored in E. coli-stimulated BEECs. In accordance with the obtained results, the use L. rhamnosus GR-1 alone could not cause the change of inflammatory factors, while L. rhamnosus GR-1 could significantly alleviate the expression of E. coli-induced inflammatory factors. Based on further study, L. rhamnosus GR-1 significantly hindered the TLR4 and MyD88 expression stimulated by E. coli. Moreover, we observed that in BEECs, L. rhamnosus GR-1 could inhibit the E. coli-elicited expressions of pathway proteins that are associated with NF-κB and MAPKs. Briefly, L. rhamnosus GR-1 can effectively protect against E. coli-induced inflammatory response that may be closely related to the inhibition of TLR4 and MyD88 stimulating NF-κB and MAPKs.