Circulating CD4+ CD25hiCD127lo Regulatory T-Cell Levels Do Not Reflect the Extent or Severity of Carotid and Coronary Atherosclerosis
Circulating CD4+ CD25hiCD127lo Regulatory T-Cell Levels Do Not Reflect the Extent or Severity of Carotid and Coronary Atherosclerosis
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DOI：
10.1161/atvbaha.110.206813
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发表时间：
2010-09-01
影响因子：
8.7
通讯作者：
Norata, Giuseppe Danilo
中科院分区：
文献类型：
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作者：
Ammirati, Enrico;Cianflone, Domenico;Norata, Giuseppe Danilo
调节性T（Treg）细胞在实验性动脉粥样硬化中起保护作用。在本研究中，我们调查了循环Treg细胞水平是否与颈动脉和冠状动脉粥样硬化的程度有关。方法和结果-我们研究了两个不同的人群：（1）113名受试者，选自自由生活的人群（颈动脉研究），其中我们测量了颈总动脉的内膜中层厚度，作为初始动脉粥样硬化的替代标志物;对照组75例，冠心病组125例，其中慢性稳定型心绞痛36例，非ST段抬高型急性冠脉综合征50例，ST段抬高型急性心肌梗死39例。通过流式细胞术（Treg细胞鉴定为CD 3（+）CD 4（+）CD 25（高）CD 127（低））和叉头盒P3或Treg相关细胞因子白细胞介素10的mRNA表达评估Treg细胞水平。在颈动脉研究中，没有观察到Treg细胞水平和内膜中层厚度之间的相关性。比较一个亚组患者（n=65）的快速与缓慢内膜-中膜厚度进展者，未观察到Treg细胞水平差异，其中可获得6年内膜-中膜厚度进展的前瞻性数据。在冠状动脉组中，慢性稳定型心绞痛患者的Treg细胞水平没有改变。相比之下，nonunivocal的变化，观察急性冠状动脉综合征患者（与Treg细胞增加ST段抬高急性心肌梗死和Treg细胞减少非ST段抬高急性冠状动脉综合征patients）。结论-结果表明，基于流式细胞术或mRNA评估的循环Treg细胞水平的测定不是动脉粥样硬化的程度或严重程度的有用指标。（Arterioscler Thromb Vasc Biol.2010; 30：1832-1841.）
Objective-Regulatory T (Treg) cells play a protective role in experimental atherosclerosis. In the present study, we investigated whether the levels of circulating Treg cells relate to the degree of atherosclerosis in carotid and coronary arteries.Methods and Results-We studied 2 distinct populations: (1) 113 subjects, selected from a free-living population (carotid study), in which we measured the intima-media thickness of the common carotid artery, as a surrogate marker of initial atherosclerosis; and (2) 75 controls and 125 patients with coronary artery disease (coronary study): 36 with chronic stable angina, 50 with non-ST-elevation acute coronary syndrome, 39 with ST-elevation acute myocardial infarction. Treg-cell levels were evaluated by flow cytometry (Treg cells identified as CD3(+) CD4(+) CD25(high)CD127(low)) and by mRNA expression of forkhead box P3 or of Treg-associated cytokine interleukin 10. In the carotid study, no correlation was observed between Treg-cell levels and intima-media thickness. No differences in Treg-cell levels were observed comparing rapid versus slow intima-media thickness progressors from a subgroup of patients (n=65), in which prospective data on 6-year intima-media thickness progression were available. In the coronary group, Treg-cell levels were not altered in chronic stable angina patients. In contrast, nonunivocal variations were observed in patients suffering an acute coronary syndrome (with a Treg-cell increase in ST-elevation acute myocardial infarction and a Treg-cell decrease in non-ST-elevation acute coronary syndrome patients).Conclusion-The results suggest that determination of circulating Treg-cell levels based on flow cytometry or mRNA assessment is not a useful indicator of the extent or severity of atherosclerosis. (Arterioscler Thromb Vasc Biol. 2010; 30: 1832-1841.)