Miniaturized side-viewing imaging probe for fluorescence lifetime imaging (FLIM): validation with fluorescence dyes, tissue structural proteins and tissue specimens
Miniaturized side-viewing imaging probe for fluorescence lifetime imaging (FLIM): validation with fluorescence dyes, tissue structural proteins and tissue specimens
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DOI：
10.1088/1367-2630/9/5/127
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发表时间：
2007-05-17
影响因子：
3.3
通讯作者：
Marcu, L.
中科院分区：
文献类型：
--
作者：
Elson, D. S.;Jo, J. A.;Marcu, L.
我们报告了一种侧视光纤内窥镜，它与血管内成像和荧光寿命成像显微镜（FLIM）兼容。该仪器已通过测试与荧光染料和胶原蛋白和弹性蛋白粉末使用Laguerre展开去卷积技术来计算荧光寿命进行验证。该仪器还在新鲜切除的未染色的动物血管组织上进行了测试。
We report a side viewing fibre-based endoscope that is compatible with intravascular imaging and fluorescence lifetime imaging microscopy (FLIM). The instrument has been validated through testing with fluorescent dyes and collagen and elastin powders using the Laguerre expansion deconvolution technique to calculate the fluorescence lifetimes. The instrument has also been tested on freshly excised unstained animal vascular tissues.