Experimental 70% hydrofluoric acid burns: histological observations in an established human skin explants ex vivo model
Experimental 70% hydrofluoric acid burns: histological observations in an established human skin explants ex vivo model
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实验%2070%%20氢氟%20酸%20烧伤：%20组织学%20观察%20in%20an%20建立%20人类%20皮肤%20外植体%20ex%20vivo%20模型
DOI：
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发表时间：
2010
影响因子：
1.6
通讯作者：
H. Maibach
中科院分区：
文献类型：
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作者：
François Burgher;L. Mathieu;E. Lati;P. Gasser;L. Peno;J. Blomet;A. Hall;H. Maibach
背景：氢氟酸（HF）特别危险，因为它可能产生全身效应，并通过两种机制诱导严重皮肤坏死：腐蚀性和局部组织毒性。此外，由于它仅部分解离（pKa 3.2），因此能够深入渗透到组织中。缺乏客观表征高频扩散到人体皮肤的行为的实验研究，特别是导致严重细胞损伤的组织渗透动力学。方法/主要发现：我们使用已建立的离体人体皮肤模型描述了 HF 的皮肤效应。 70% HF 在接触表皮表面的第一分钟内开始扩散，2 分钟后到达基底层。在接下来的一分钟内，表皮被破坏，4分钟后真皮乳头层出现病变。不久之后，上层网状真皮出现损伤。因此，70% HF 只需接触 5 分钟即可完全渗透人体皮肤外植体。该实验具有可重复性，并证实了之前的研究和意外接触高频暴露的临床效果。结论/意义：这项研究表明，HF化学皮肤暴露的管理只需几分钟的时间，特别是对于初始净化而言。这些实验观察结果可用于客观比较皮肤净化方法。进一步的研究应该有助于证实这些初步结果。
Background: Hydrofluoric acid (HF) is particularly dangerous due to the potential for systemic effects and induction of severe skin necrosis through two mechanisms: corrosiveness and local tissue toxicity. In addition, because it is only partially dissociated (pKa 3.2), it is capable of penetrating deeply into tissues. There is a lack of experimental studies that objectively characterize the behavior of HF diffusion into human skin, specifically the kinetics of tissue penetration resulting in severe cellular lesions. Methodology/principal findings: We describe the cutaneous effects of HF using an established ex vivo human skin model. The diffusion of 70% HF starts within the first minute of contact at the epidermal surface and after 2 min reaches the basal layer. In the subsequent minute, the epidermis is destroyed and lesions appear in the papillary dermis after 4 min. Soon after, damage appears in the upper reticular dermis. Thus, 70% HF needs only 5 min of contact to completely penetrate human skin explants. This experiment is reproducible and corroborates previous studies and clinical effects reported in accidental HF exposures. Conclusion/significance: This study shows that the management of HF chemical skin exposure is a question of minutes, especially for initial decontamination. These experimental observations could be useful for objectively comparing skin decontamination methods. Further studies should help to confirm these preliminary results.