SINGLE UNIT ACTIVITY OF THE SUPRACHIASMATIC NUCLEUS AND SURROUNDING NEURONS DURING THE WAKE-SLEEP CYCLE IN MICE
SINGLE UNIT ACTIVITY OF THE SUPRACHIASMATIC NUCLEUS AND SURROUNDING NEURONS DURING THE WAKE-SLEEP CYCLE IN MICE
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DOI：
10.1016/j.neuroscience.2013.12.020
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发表时间：
2014-02-28
期刊：
影响因子：
3.3
通讯作者：
Sakai, K.
中科院分区：
文献类型：
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作者：
Sakai, K.
哺乳动物下丘脑的视交叉上核（SCN）包含一个生物钟，用于定时各种神经元、内分泌和行为节律，如睡眠和觉醒的周期。利用细胞外单单元记录，我们首次在未麻醉、头部受限的小鼠中确定了单个SCN神经元在整个清醒-睡眠周期中的放电活动。根据SCN神经元在清醒-睡眠周期中有规律（I型，n = 38）或不规则（II型，n = 19）放电活动或清醒时的静止活动和睡眠时的不规则放电活动（III型，n = 22），将79个SCN神经元分为三种类型。ⅰ型和ⅱ型神经元表现为长时态动作电位，ⅲ型神经元表现为短时态或长时态动作电位。I型神经元完全以单个孤立的尖峰放电，而II型和III型神经元以单个孤立的尖峰、簇或脉冲放电。I型和II型神经元表现为清醒活跃、清醒/矛盾（或快速眼动）睡眠活跃或状态无关的活动特征，分别主要位于SCN的腹侧或背侧区域。相比之下，III型神经元表现出睡眠活动放电特征，主要位于SCN的外侧区域。大多数I型和II型神经元测试显示，在动物的眼睛上施加光后，放电率增加。在记录的289个额外的SCN神经元中，显示睡眠活跃放电特征的神经元主要位于SCN的背侧，而显示清醒活跃放电特征的神经元主要位于SCN的外侧或背侧。本研究揭示了小鼠SCN及其周围下丘脑前神经元的异质性，并提示它们的地形组织和在哺乳动物昼夜节律和睡眠和觉醒调节中的作用存在差异。(c) 2013 ibro。Elsevier Ltd.出版。版权所有。
The suprachiasmatic nucleus (SCN) of the mammalian hypothalamus contains a circadian clock for timing of diverse neuronal, endocrine, and behavioral rhythms, such as the cycle of sleep and wakefulness. Using extracellular single unit recordings, we have determined, for the first time, the discharge activity of individual SCN neurons during the complete wake-sleep cycle in non-anesthetized, head restrained mice. SCN neurons (n = 79) were divided into three types according to their regular (type I; n = 38) or irregular (type II; n = 19) discharge activity throughout the wake-sleep cycle or their quiescent activity during waking and irregular discharge activity during sleep (type III; n = 22). The type I and II neurons displayed a long-duration action potential, while the type III neurons displayed either a short-duration or long-duration action potential. The type I neurons discharged exclusively as single isolated spikes, whereas the type II and III neurons fired as single isolated spikes, clusters, or bursts. The type I and II neurons showed wake-active, wake/paradoxical (or rapid eye movement) sleep-active, or state-unrelated activity profiles and were, respectively, mainly located in the ventral or dorsal region of the SCN. In contrast, the type III neurons displayed sleep-active discharge profiles and were mainly located in the lateral region of the SCN. The majority of type I and II neurons tested showed an increase in discharge rate following application of light to the animal's eyes. Of the 289 extra-SCN neurons recorded, those displaying sleepactive discharge profiles were mainly located dorsal to the SCN, whereas those displaying wake-active discharge profiles were mainly located lateral or dorsolateral to the SCN. This study shows heterogeneity of mouse SCN and surrounding anterior hypothalamic neurons and suggests differences in their topographic organization and roles in mammalian circadian rhythms and the regulation of sleep and wakefulness. (C) 2013 IBRO. Published by Elsevier Ltd. All rights reserved.