MOLECULAR-CLONING OF NOTCH, A LOCUS AFFECTING NEUROGENESIS IN DROSOPHILA-MELANOGASTER
MOLECULAR-CLONING OF NOTCH, A LOCUS AFFECTING NEUROGENESIS IN DROSOPHILA-MELANOGASTER
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DOI：
10.1073/pnas.80.7.1977
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发表时间：
1983-01-01
期刊：
影响因子：
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通讯作者：
YEDVOBNICK, B
中科院分区：
文献类型：
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作者：
ARTAVANISTSAKONAS, S;MUSKAVITCH, MAT;YEDVOBNICK, B
就其遗传结构和发育效应而言，Notch 基因座是黑腹果蝇中最具特征的基因座之一。该位点的突变深刻影响早期胚胎的分化。使用涉及Notch基因座和先前克隆的序列的倒位，从包含80kb DNA的Notch区域(3C7)的染色体片段被分离出来。根据突变型和野生型 DNA 之间的比较，克隆序列被定位在 Notch 遗传图谱中； .apprx 的区域。定义了 40 kb，其中与迄今为止映射的所有 Notch 等位基因相关的结构损伤似乎存在。在个体发育过程中检查了克隆序列的转录活性，发现了单一大小类别的聚（A）+RNA，长10.5 kb，与该40 kb区域内的序列同源。克隆了属于 Notch 基因座的 DNA 序列，10.5 kb poly(A)+ RNA 对于野生型 Notch 功能至关重要。这些结构和转录数据根据Notch基因座的现有遗传和发育特征进行讨论。
The Notch locus is one of the best characterized loci in D. melanogaster in terms of its genetic structure and developmental effects. Mutations in this locus profoundly affect the differentiation of the early embryo. Using an inversion involving the Notch locus and previously cloned sequences, chromosomal segments from the Notch region (3C7) encompassing 80 kilobases (kb) of DNA were isolated. Based on comparison between mutant and wild-type DNA, cloned sequences were positioned within the Notch genetic map; a region of .apprx. 40 kb was defined within which the structural lesions correlating with all Notch alleles mapped to date appear to reside. The transcriptional activity of the cloned sequences was examined during ontogeny and find a single size class of poly(A)+RNA, 10.5 kb long, that is homologous to sequences within this 40 kb region. DNA sequences belonging to the Notch locus were cloned and the 10.5 kb poly(A)+ RNA is essential for wild-type Notch function. These structural and transcriptional data are discussed in light of the existing genetic and developmental characterization of the Notch locus.