Comparison of 250 MHz electron spin echo and continuous wave oxygen EPR imaging methods for in vivo applications
Comparison of 250 MHz electron spin echo and continuous wave oxygen EPR imaging methods for in vivo applications
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DOI：
10.1118/1.3555297
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发表时间：
2011-04-01
期刊：
影响因子：
3.8
通讯作者：
Halpern, Howard J.
中科院分区：
文献类型：
--
作者：
Epel, Boris;Sundramoorthy, Subramanian V.;Halpern, Howard J.
目的：作者比较了两种电子顺磁共振成像模式在250 MHz，以确定这些模式的优点和缺点，在体内氧imaging.Methods：电子自旋回波（ESE）和连续波（CW）的方法，以获得三维图像的窄线宽，水溶性，无毒的氧敏感三苯甲基分子OX 063在体外和体内。作者还研究了从同一动物静脉注射三苯甲基自旋探针，以确定时间的稳定性的methods.Results：phanomaly与不同的氧浓度的研究显示了三倍的优势，ESE方法在减少成像时间和更精确的氧分辨率小于70毫升氧分压的样品。在100 kHz以上，CW表现更好。两种方法产生的图像显示pO（2）分布具有相似的平均值。然而，ESE图像在低pO（2）区域表现出上级性能，而在高pO（2）区域丢失体素。ESE在肿瘤缺氧研究中具有上级优势。(C)2011年美国医学物理学家协会。[DOI：10.1118/1.3555297]
Purpose: The authors compare two electron paramagnetic resonance imaging modalities at 250 MHz to determine advantages and disadvantages of those modalities for in vivo oxygen imaging.Methods: Electron spin echo (ESE) and continuous wave (CW) methodologies were used to obtain three-dimensional images of a narrow linewidth, water soluble, nontoxic oxygen-sensitive trityl molecule OX063 in vitro and in vivo. The authors also examined sequential images obtained from the same animal injected intravenously with trityl spin probe to determine temporal stability of methodologies.Results: A study of phantoms with different oxygen concentrations revealed a threefold advantage of the ESE methodology in terms of reduced imaging time and more precise oxygen resolution for samples with less than 70 torr oxygen partial pressure. Above similar to 100 torr, CW performed better. The images produced by both methodologies showed pO(2) distributions with similar mean values. However, ESE images demonstrated superior performance in low pO(2) regions while missing voxels in high pO(2) regions.Conclusions: ESE and CW have different areas of applicability. ESE is superior for hypoxia studies in tumors. (C) 2011 American Association of Physicists in Medicine. [DOI: 10.1118/1.3555297]