MC540 and upconverting nanocrystal coloaded polymeric liposome for near-infrared light-triggered photodynamic therapy and cell fluorescent imaging.
MC540 and upconverting nanocrystal coloaded polymeric liposome for near-infrared light-triggered photodynamic therapy and cell fluorescent imaging.
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DOI：
10.1021/am500097f
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发表时间：
2014-02
影响因子：
9.5
通讯作者：
Hanjie Wang;Zhongyun Liu;Sheng Wang;Chunhong Dong;Xiaoqun Gong;P. Zhao;Jin Chang
中科院分区：
文献类型：
--
作者：
Hanjie Wang;Zhongyun Liu;Sheng Wang;Chunhong Dong;Xiaoqun Gong;P. Zhao;Jin Chang
在临床上，光动力学疗法（PDT）在深部组织中的应用受到激活光敏剂（PS）所需的可见光穿透深度的限制。本文设计并成功构建了一种Mercury 540（MC 540）和上转换纳米颗粒（UCN）共载的功能性高分子脂质体纳米载体（MC 540 + UCN）/FPL，以解决PDT治疗中的这一问题。与传统的UCN直接吸附PS的方法相比，UCN与高分子脂质体的结合具有独特的优势。UCN核心作为传感器可以将深穿透的近红外光转换为可见光，以激活MC 540。叶酸修饰的功能性高分子脂质体壳层作为纳米屏障，可以保持UCN和MC 540的稳定，保护它们免受攻击，并帮助它们进入细胞。结果表明，（MC 540 + UCN）/FPL为单分散纳米球，平均粒径为26 nm。MC 540可以通过上转换UCN发射的荧光而被激活以成功地产生单线态氧。叶酸修饰（MC 540 + UCN）/FPL后，细胞摄取效率明显提高。更有趣的是，在PDT效果测试中，（MC 540 + UCN）/FPL纳米载体通过锚定靶向叶酸和反式激活转导肽进一步提高了对肿瘤细胞的抑制效果。我们的数据表明，（MC 540 + UCN）/FPL纳米载体可能是一个有用的纳米平台，为未来的PDT治疗在深度癌症治疗的基础上的上转换机制。
In clinic, the application of photodynamic therapy (PDT) in deep tissue is severely constrained by the limited penetration depth of visible light needed for activating the photosensitizer (PS). In this Article, a merocyanine 540 (MC540) and upconverting nanoparticle (UCN) coloaded functional polymeric liposome nanocarrier, (MC540 + UCN)/FPL, was designed and constructed successfully for solving this problem in PDT. Compared with the conventional approaches using UCNs absorbing PSs directly, the combination of UCN and polymeric liposome has unique advantages. The UCN core as a transducer can convert deep-penetrating near-infrared light to visible light for activating MC540. The functional polymeric liposome shell decorated with folate as a nanoshield can keep the UCN and MC540 stable, protect them from being attacked, and help them get into cells. The results show that (MC540 + UCN)/FPL is an individual nanosphere with an average size of 26 nm. MC540 can be activated to produce singlet oxygen successfully by upconverting fluorescence emitted from UCNs. After (MC540 + UCN)/FPL was modified with folate, the cell uptake efficiency increased obviously. More interestingly, in the PDT effect test, the (MC540 + UCN)/FPL nanocarrier further improved the inhibition effect on tumor cells by anchoring targeting folate and transactivating transduction peptide. Our data suggest that the (MC540 + UCN)/FPL nanocarrier may be a useful nanoplatform for future PDT treatment in deep-cancer therapy based on upconversion mechanism.