REGIONAL STUDIES OF CATECHOLAMINES IN RAT BRAIN .I. DISPOSITION OF [3H]NOREPINEPHRINE [3H]DOPAMINE AND [3H]DOPA IN VARIOUS REGIONS OF BRAIN
REGIONAL STUDIES OF CATECHOLAMINES IN RAT BRAIN .I. DISPOSITION OF [3H]NOREPINEPHRINE [3H]DOPAMINE AND [3H]DOPA IN VARIOUS REGIONS OF BRAIN
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DOI：
10.1111/j.1471-4159.1966.tb09873.x
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发表时间：
1966-01-01
影响因子：
4.7
通讯作者：
IVERSEN, LL
中科院分区：
文献类型：
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作者：
GLOWINSKI, J;IVERSEN, LL
描述了将大鼠大脑解剖成以下区域的方法：小脑、延髓、下丘脑、纹状体、中脑、海马和皮质。测定各区域内源性去甲肾上腺素含量；下丘脑浓度最高，小脑浓度最低。向大脑侧脑室注射少量氚化去甲肾上腺素、二羟基苯丙氨酸（DOPA）或二羟基苯乙胺（多巴胺）后，在不同时间检查7个区域中放射性儿茶酚胺的分布。给予[H3]去甲肾上腺素后，标记胺在不同脑区的积累与内源性去甲肾上腺素的区域分布相关，在下丘脑中发现最高水平。然而，在纹状体[长划线]区域也发现了高水平的[H3]去甲肾上腺素，该区域含有高浓度的内源性多巴胺，但仅含有低浓度的去甲肾上腺素。标记的去甲肾上腺素在不同脑区的代谢没有质的差异，但代谢物存在量的差异。在大多数区域，除小脑和皮质外，代谢物与未变化的去甲肾上腺素的比例随着时间的推移而减少。未变化的[H3]去甲肾上腺素以不同的速度从不同的大脑区域消失。给予[H3]多巴胺后，大多数脑区迅速形成[H3]-去甲肾上腺素，但标记去甲肾上腺素的最大水平在不同时间达到。在纹状体中，只能检测到极少量的标记去甲肾上腺素，但在该区域发现了最高水平的[H3]多巴胺。多巴胺后氚化儿茶酚胺的区域分布与[H3]去甲肾上腺素后发现的相似。注射[H3]多巴后，转化为儿茶酚胺的程度有限；注射多巴后5小时，大脑所有区域的[H3]多巴胺水平大大超过[H3]去甲肾上腺素。
A method is described for the dissection of the rat brain into the following regions: cerebellum, medulla oblongata, hypothalamus, striatum, midbrain, hippocampus, and cortex. The endogenous norepinephrine content of each region was measured; highest concentrations were found in the hypothalamus and lowest in the cerebellum. The distribution of radioactive catecholamines in the 7 regions was examined at different times after the injection of small amounts of tritiated norepinephrine, di-hydroxyphenylalanine (DOPA) or dihydroxyphenylethylamine (dopamine) into the lateral ventricle of the brain. After the administration of [H3] norepinephrine, the accumulation of the labelled amine in various brain regions correlated with the regional distribution of endogenous norepinephrine, highest levels being found in the hypothalamus. High levels of [H3]noreplnephrine were, however, also found in the striatum[long dash]a region which contained a high concentration of endogenous dopamine but only a low concentration of norepinephrine. There were no qualitative differences in the metabolism of labelled norepinephrine in the different brain regions, though quantitative differences in metabolites occurred. In most regions the proportion of metabolites to unchanged norepinephrine decreased with time except in cerebellum and cortex. Unchanged [H3]norepinephrine disappeared at different rates from the various brain areas. After the administration of [H3]dopamine there was a rapid formation of [H3]-norepinephrine inmost brain regions, but maximum levels of labelled norepinephrine were attained at different times. In the striatum only very small amounts of labelled norepinephrine could be detected, but the highest levels of [H3]dopamine were found in this region. The regional distribution of tritiated catecholamines after dopamine was similar to that found after [H3]norepinephrine. After the injection of [H3] DOPA there was a limited conversion to catecholamines; in all areas of the brain the levels of [H3]dopamine greatly exceeded those of [H3]noreptnephrine 5 hr after the injection of DOPA.