Human osteoblasts within soft peptide hydrogels promote mineralisation in vitro.
Human osteoblasts within soft peptide hydrogels promote mineralisation in vitro.
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DOI：
10.1177/2041731414539344
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发表时间：
2014
影响因子：
8.2
通讯作者：
Miller AF
中科院分区：
文献类型：
--
作者：
Castillo Diaz LA;Saiani A;Gough JE;Miller AF
为细胞培养提供三维支持网络的生物材料正在被开发用于广泛的组织工程应用，包括骨再生。这项研究探索了多功能离子互补肽FEFEFKFK的潜力，其目的是使该肽自发地自组装成富含β片层的纤维，随后自缔合形成自支撑水凝胶。通过简单的活/死细胞测定，我们证明了3wt%的水凝胶对于支持成骨细胞是最佳的。我们继续表明，这些细胞不仅在三维水凝胶中存活，而且它们还增殖并产生成骨关键蛋白，也就是说，它们在这里探索的14天内表现得像体内骨细胞。当细胞存在时，凝胶弹性随时间增加-与对照样品中的减少相比-表明基质在整个肽支架中的沉积。此外，沉积了大量的磷酸钙。总的来说，这些数据表明，离子互补八肽提供了一个合适的三维环境成骨细胞的功能。
Biomaterials that provide three-dimensional support networks for the culture of cells are being developed for a wide range of tissue engineering applications including the regeneration of bone. This study explores the potential of the versatile ionic-complementary peptide, FEFEFKFK, for such a purpose as this peptide spontaneously self-assembles into β-sheet-rich fibres that subsequently self-associate to form self-supporting hydrogels. Via simple live/dead cell assays, we demonstrated that 3 wt% hydrogels were optimal for the support of osteoblast cells. We went on to show that these cells are not only viable within the three-dimensional hydrogel but they also proliferate and produce osteogenic key proteins, that is, they behave like in vivo bone cells, over the 14-day period explored here. The gel elasticity increased over time when cells were present – in comparison to a decrease in control samples – indicating the deposition of matrix throughout the peptide scaffold. Moreover, significant quantities of calcium phosphate were deposited. Collectively, these data demonstrate that ionic-complementary octapeptides offer a suitable three-dimensional environment for osteoblastic cell function.