Methyl jasmonate primes defense responses against Botrytis cinerea and reduces disease development in harvested table grapes
Methyl jasmonate primes defense responses against Botrytis cinerea and reduces disease development in harvested table grapes
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茉莉酸甲酯启动针对灰葡萄孢的防御反应并减少收获的鲜食葡萄中的疾病发展
DOI：
10.1016/j.scienta.2015.06.015
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发表时间：
2015-08-31
影响因子：
4.3
通讯作者：
Zheng, Yonghua
中科院分区：
文献类型：
--
作者：
Jiang, Lulu;Jin, Peng;Zheng, Yonghua
研究了10 μmol/L茉莉酸甲酯(MeJA)采后处理对控制鲜食葡萄灰霉病灰霉病的效果及其可能的作用机制。结果表明，MeJA 处理显着降低了葡萄灰霉病的发生率和发展。同时，MeJA处理显着增强超氧化物歧化酶（SOD）和过氧化氢酶（CAT）活性，降低抗坏血酸过氧化物酶活性，促进H2O2积累，提高2,2-二苯基-1-三硝基苯肼（DPPH）自由基清除活性。 MeJA 处理还显着增强了防御相关酶的活性，包括几丁质酶 (CHI)、β-1,3-葡聚糖酶 (GNS)、苯丙氨酸解氨酶 (PAL)、多酚氧化酶 (PPO) 和过氧化物酶 (POD)。此外，与仅用MeJA处理或接种灰霉病菌的果实相比，编码CHI、GNS、PAL、SOD和CAT的防御相关基因的转录物仅在用MeJA预处理然后接种灰霉病菌的果实中显着增强。这些结果表明，10 μmol/L MeJA 处理可引发防御反应，增强对灰霉病的抗病性，从而减少收获的鲜食葡萄中的灰霉病。 (C) 2015 Elsevier B.V. 保留所有权利。
The effect of a postharvest treatment with 10 mu mol/L of methyl jasmonate (MeJA) on controlling gray mold decay caused by Botrytis cinerea in table grapes and the possible action mechanisms were investigated. The results indicated that MeJA treatment significantly reduced the incidence and development of gray mold decay in grapes. Meanwhile, MeJA treatment markedly enhanced activities of superoxide dismutase (SOD) and catalase (CAT), decreased ascorbate peroxidase activity, promoted the accumulation of H2O2 and improved the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity. MeJA treatment also significantly enhanced activities of defense related enzymes including chitinase (CHI), beta-1,3-glucanase (GNS), phenylalanine ammonia-lyase (PAL), polyphenol oxidase (PPO) and peroxidase (POD). Moreover, transcripts of defense related genes encoding CHI, GNS, PAL, SOD and CAT were only significantly enhanced in fruit pre-treated with MeJA and then inoculated with B. cinerea compared with those that were only treated with MeJA or inoculated with B. cinerea. These results suggested that 10 mu mol/L of MeJA treatment primes defense responses for enhanced disease resistance against Botrytis cinerea and thus reducing gray mold decay in harvested table grapes. (C) 2015 Elsevier B.V. All rights reserved.