A new subtype (subgenotype) Ac (A3) of hepatitis B virus and recombination between genotypes A and E in Cameroon
A new subtype (subgenotype) Ac (A3) of hepatitis B virus and recombination between genotypes A and E in Cameroon
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DOI：
10.1099/vir.0.80922-0
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发表时间：
2005-07-01
影响因子：
3.8
通讯作者：
Mizokami, M
中科院分区：
文献类型：
--
作者：
Kurbanov, F;Tanaka, Y;Mizokami, M
对西非喀麦隆两个不同人群（俾格米人和班图人）的血样（n = 544）进行了分析。血清学检测结果表明，班图人抗丙型肝炎病毒（HCV）感染率（20 - 3%）高于俾格米人（2（.）3%，P <0.0001），而B型肝炎病毒（HBV）血清学标志物在两个人群中的分布同样高：总共9（.）4、17（.）3和86（.）HBsAg、抗-HBs、抗-HBc分别为8 0/B。HBV基因型A（HBV/A）和HBV/E占优势（43例）。在两个人群中各占5%），少数人群（13%）发现HBV/D。对9例患者（5例HBV/A和4例HBV/E）的preS/S区进行了测序，并对6例患者（4例HBV/A和2例HBV/E）的全基因组进行了测序。随后的系统发育分析显示，HBV/A株与先前描述的亚型（亚基因型）Ae（A2）和Aa（A1）不同，在preS/S区域，它们与先前报道的喀麦隆序列聚类。基于Aa（A1）和Ae（A2）的核粒差异，在全基因组中超过4%，建议Escheronian株代表一种新的亚型（亚基因型），命名为HBV/Ac（A3）。高（3）9%）的核粒差异表明，该亚型（亚基因型）在喀麦隆人群中具有较长的自然历史。发现其中一株HBV/Ac（A3）是在聚合酶逆转录酶结构域中具有HBV/E特异性序列的重组体。需要进一步的队列研究来评估HBV/Ac（A3）及其重组形式的详细流行病学、病毒学和临床特征。
Blood samples (n=544) from two different populations (Pygmies and Bantus) in Cameroon, West Africa, were analysed. Serological tests indicated that the anti-hepatitis C virus (HCV) prevalence in Bantus (20-3%) was higher than that in Pygmies (2(.)3%, P < 0.0001), whereas the distribution of hepatitis B virus (HBV) serological markers was equally high in both populations: in total, 9(.)4, 17(.)3 and 86(.)8 0/b for HBsAg, anti-HBs and anti-HBc, respectively. HBV genotype A (HBV/A) and HBV/E were predominant (43(.)5% each) in both populations, and HBV/D was found in a minority (13%). The preS/S region was sequenced in nine cases (five HBV/A and four HBV/E) and the complete genome in six cases (four HBV/A and two HBV/E). Subsequent phylogenetic analysis revealed that the HBV/A strains were distinct from the subtypes (subgenotypes) described previously, Ae (A2) and Aa (A1), and in the preS/S region they clustered with previously reported sequences from Cameroon. Based on the nucleoticle difference from Aa (A1) and Ae (A2), more than 4% in the complete genome, the Cameroonian strains were suggested to represent a new subtype (subgenotype), designated HBV/Ac (A3). A high (3(.)9 %) nucleoticle divergence in HBV/Ac (A3) strains suggested that the subtype (subgenotype) has a long natural history in the population of Cameroon. One of the HBV/Ac (A3) strains was found to be a recombinant with an HBV/E-specific sequence in the polymerase reverse transcriptase domain. Further cohort studies will be required to assess detailed epidemiological, virological and clinical characteristics of HBV/Ac (A3), as well as its recombinant form.