A HIGHLY CONSERVED AMINO-ACID-SEQUENCE IN THROMBOSPONDIN, PROPERDIN AND IN PROTEINS FROM SPOROZOITES AND BLOOD STAGES OF A HUMAN MALARIA PARASITE
A HIGHLY CONSERVED AMINO-ACID-SEQUENCE IN THROMBOSPONDIN, PROPERDIN AND IN PROTEINS FROM SPOROZOITES AND BLOOD STAGES OF A HUMAN MALARIA PARASITE
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DOI：
10.1038/335079a0
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发表时间：
1988-09-01
期刊：
影响因子：
64.8
通讯作者：
WEATHERALL, DJ
中科院分区：
文献类型：
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作者：
ROBSON, KJH;HALL, JRS;WEATHERALL, DJ
作为基因克隆和DNA测序的结果，在细胞-细胞识别领域出现了几个基因家族。这些蛋白包括免疫球蛋白、整合素、某些细胞外糖蛋白1和一系列功能无关的蛋白，其中包括因子B2。我们报道了恶性疟原虫一个基因的克隆和序列测定，该基因编码一种我们称为凝血酶反应蛋白相关匿名蛋白(TRAP)的蛋白，该蛋白具有与其他功能良好的蛋白相同的某些序列基序。最有意义的同源性是基于序列Trp-Ser-Pro-Cys-Ser-Val-Thr-Cys-Gly(WSPCSVTCG)，存在于血栓反应蛋白(TSP)3区的3个拷贝，属性4(P)的6个拷贝和所有已测序的环子孢子(CS)蛋白5-10中的一个拷贝。TRAP还与某些胞外糖蛋白共享，包括TSP，细胞识别信号Arg-Gly-Asp(RGD)11，已被证明在几种胞外糖蛋白与整合素超家族成员的相互作用中起关键作用。与CS蛋白不同，TRAP在寄生虫生命周期的红细胞阶段表达。
As a consequence of gene cloning and DNA sequencing several gene families are emerging in the field of cell–cell recognition. These include immunoglobulins, integrins, certain extracellular glycoproteins1and a family of functionally unrelated proteins which include factor B2. We report here the cloning and sequencing of a gene fromPlasmodium falciparum, coding for a protein we call thrombospondin related anonymous protein (TRAP), which shares certain sequence motifs common to other well-characterized proteins. The most significant homology is based around the sequence Trp-Ser-Pro-Cys-Ser-Val-Thr-Cys-Gly (WSPCSVTCG), present in three copies in region I of thrombospondin (TSP)3, six copies in properdin4(P) and one copy in all the circumsporozoite (CS) proteins5–10sequenced so far. TRAP also shares with certain extracellular glycoproteins, including TSP, the cell-recognition signal Arg-Gly-Asp (RGD)11, which has been shown to be crucial in the interaction of several extracellular glycoproteins with members of the integrin superfamily. Unlike the CS protein, TRAP is expressed during the erythrocytic stage of the parasite life cycle.