Interaction of aliphatic amino acids with zwitterionic and charged lipid membranes: hydration and dehydration phenomena
Interaction of aliphatic amino acids with zwitterionic and charged lipid membranes: hydration and dehydration phenomena
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DOI：
10.1039/c9cp06188f
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发表时间：
2020-02-14
影响因子：
3.3
通讯作者：
Chakraborty, Anjan
中科院分区：
文献类型：
--
作者：
Kanwa, Nishu;Kanti, De Soumya;Chakraborty, Anjan
在目前的贡献，我们调查的相互作用的脂双层膜的不同电荷和不同的相态与脂肪族氨基酸的不同电荷（天冬氨酸，谷氨酸，精氨酸和赖氨酸）和疏水性（丝氨酸，亮氨酸和缬氨酸）的稳态和时间分辨光谱技术，动态光散射（DLS）测量和共聚焦成像（CLSM）。研究表明，带负电荷的氨基酸，如天冬氨酸和谷氨酸与脂质膜，特别是与带负电荷的脂质膜，通过稳定它们的凝胶相强烈的相互作用。另一方面，带正电荷的氨基酸在膜中引起水合作用。我们解释了这一独特的观察，在最近的计算机模拟光的脂膜和溶剂化和去溶剂化过程中的附近的氨基酸的pK（a）的转变。我们还发现，氢键起着重要的作用，在管理脂肪族氨基酸与两性离子脂质膜的相互作用。在末端碳上带有羟基的极性更强的丝氨酸与其类似物亮氨酸和缬氨酸（本质上是疏水性的）相比，提供了与脂质双层膜更强的相互作用。
In the present contribution, we investigate the interactions of lipid bilayer membranes of different charges and different phase states with aliphatic amino acids of varying charge (aspartic acid, glutamic acid, arginine and lysine) and hydrophobicity (serine, leucine and valine) by steady state and time-resolved spectroscopic techniques, dynamic light scattering (DLS) measurements and confocal imaging (CLSM). The study reveals that negatively charged amino acids such as aspartic acid and glutamic acid interact strongly with the lipid membranes particularly with negatively charged lipid membranes by stabilizing their gel phase. On the other hand, positively charged amino acids bring in hydration in the membranes. We explain this unique observation by the shift in pK(a) of amino acids in the vicinity of the lipid membranes and solvation and desolvation processes in the light of recent computer simulations. We also find that hydrogen bonding plays a significant role in governing the interaction of aliphatic amino acids with zwitterionic lipid membranes. The more polar serine bearing a hydroxyl group at the terminal carbon offers a stronger interaction with the lipid bilayer membranes as compared to its analogues leucine and valine, which are hydrophobic in nature.