EFFECT OF ALPHA-CHLORALOSE, HALOTHANE, PENTOBARBITAL AND NITROUS-OXIDE ANESTHESIA ON METABOLIC COUPLING IN SOMATOSENSORY CORTEX OF RAT
EFFECT OF ALPHA-CHLORALOSE, HALOTHANE, PENTOBARBITAL AND NITROUS-OXIDE ANESTHESIA ON METABOLIC COUPLING IN SOMATOSENSORY CORTEX OF RAT
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DOI：
10.1111/j.1399-6576.1992.tb03474.x
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发表时间：
1992-05-01
影响因子：
2.1
通讯作者：
HOSSMANN, KA
中科院分区：
文献类型：
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作者：
UEKI, M;MIES, G;HOSSMANN, KA
研究了不同麻醉剂对单侧躯体感觉刺激大鼠大脑皮层功能代谢偶联的影响。脑局部葡萄糖代谢率(CMR(GLC))采用2-脱氧葡萄糖放射自显影方法测定，体感兴奋通过电刺激左前爪实现。在用70%一氧化二氮、0.5%氟烷/70%一氧化二氮或40 mg/kg戊巴比妥钠处理的动物中，尽管产生了初级诱发皮层电位，但体感皮层的CMR(GLC)没有改变。相反，80 mg/kg的α-氯醛糖麻醉后，初级躯体感觉皮层的CMR(GLC)从52.1+/-18.3增加到73.1+/-18.9-mU·mol/100g/min(平均值+/-S.D.)。代谢激活严格局限于躯体感觉皮质的前肢(FL)区，在I、II和IV层表现出最大的激活。在外科剂量的氯醛糖下保存功能-代谢偶联，使这种麻醉剂特别适合于在实验要求排除使用未麻醉动物的条件下研究偶联过程。
The effect of various anesthetics on the functional-metabolic coupling of cerebral cortex was studied in rats submitted to unilateral somatosensory stimulation. The regional cerebral metabolic rate of glucose (CMR(glc)) was measured autoradiographically using the 2-deoxyglucose method, and somatosensory activation was carried out by electrical stimulation of the left forepaw. In animals treated with 70% nitrous oxide, 0.5% halothane/70% nitrous oxide or 40 mg/kg pentobarbital, CMR(glc) of somatosensory cortex did not change despite generation of primary evoked cortical potentials. Anesthesia with 80 mg/kg alpha-chloralose, in contrast, led to a focal increase of CMR(glc) in the primary somatosensory cortex from 52.1 +/- 18.3 to 73.1 +/- 18.9-mu-mol/100 g/min (means +/- s.d.). Metabolic activation was strictly confined to the forelimb (FL) area of somatosensory cortex, and it exhibited a laminar pattern with maximal activation in layers I, II and IV. The preservation of functional-metabolic coupling under a surgical dose of chloralose renders this anesthetic particularly suited for the investigation of coupling processes under conditions where the experimental requirements preclude the use of unanaesthetized animals.