A comparative study of apoptosis and proliferation in germinoma and glioblastoma
A comparative study of apoptosis and proliferation in germinoma and glioblastoma
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DOI：
10.1093/neuonc/2.2.96
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发表时间：
2000-04-01
期刊：
影响因子：
15.9
通讯作者：
Fukui, M
中科院分区：
文献类型：
--
作者：
Mizoguchi, M;Inamura, T;Fukui, M
颅内生殖细胞瘤在放疗和化疗治疗时预后相对较好，而胶质母细胞瘤无论采用何种治疗，预后均较差。细胞增殖和细胞死亡是肿瘤生长中相反的过程，肿瘤进展反映了增殖细胞和凋亡细胞之间的平衡。我们用MIB-1染色和缺口末端标记法检测了13例生殖细胞瘤和11例胶质母细胞瘤的细胞增殖和细胞死亡情况，并用免疫组化法检测了调控细胞凋亡的BAX和Bcl-2的表达。虽然生殖细胞瘤显示出类似于胶质母细胞瘤的强MIB-1免疫染色，但生殖细胞瘤中包含了更多的凋亡细胞。生殖细胞瘤的凋亡率与MIB-1标记指数的比值为72.9 +/- 36.9（平均值+/- SD），与胶质母细胞瘤相比，该比值更高，具有统计学显著性（14.5 +/- 11.2; P < 0.01）。此外，生殖细胞瘤表现出更大的表达BAX比胶质母细胞瘤，而Bcl-2的表达是弱的两种肿瘤类型。这些凋亡相关蛋白的比较显示，BAX在生殖细胞瘤中的免疫反应性相对高于胶质母细胞瘤（P < 0.01），与生殖细胞瘤组织中鉴定的大量凋亡细胞相对应。这些发现可能解释了生殖细胞瘤和胶质母细胞瘤之间的预后差异，根据MIB-1免疫染色，两者具有相似的增殖潜力。在预测颅内肿瘤患者的预后时，应考虑细胞增殖和死亡之间的平衡。
Intracranial germinoma has a relatively good prognosis when treated with radiotherapy and chemotherapy, whereas glioblastoma has a poor prognosis irrespective of these treatments. Cell proliferation and cell death are opposing processes in tumor growth, with tumor progression reflecting the balance between proliferating and apoptotic cells. We investigated cell proliferation and cell death using MIB-1 staining and nick-end labeling in 13 germinomas in comparison with 11 glioblastomas, Expression of BAX and Bcl-2, which regulate apoptosis, were studied by immunohistochemistry. Although germinomas showed strong MIB-1 immunostaining similar to that seen in glioblastomas, germinomas included significantly more apoptotic cells. The ratio of apoptotic ratio to MIB-1 labeling index for germinomas was 72.9 +/- 36.9 (mean +/- SD), a higher, statistically significant ratio as compared with glioblastomas (14.5 +/- 11.2; P < 0.01). Furthermore, germinomas showed greater expression of BAX than did glioblastomas, while the expression of Bcl-2 was weak in both tumor types. A comparison of these apoptotic-related proteins showed that immunoreactivity for BAX was relatively higher in germinomas than in glioblastomas (P < 0.01), corresponding well to numerous apoptotic cells identified in germinoma tissues. These findings may account for the prognostic difference between germinoma and glioblastoma in the face of a similar proliferation potential according to MIB-1 immunostaining. The balance between cell proliferation and death should be considered when predicting outcomes in patients with intracranial tumors.