Culture Independent Raman Spectroscopic Identification of Urinary Tract Infection Pathogens: A Proof of Principle Study
Culture Independent Raman Spectroscopic Identification of Urinary Tract Infection Pathogens: A Proof of Principle Study
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DOI：
10.1021/ac401806f
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发表时间：
2013-10-15
影响因子：
7.4
通讯作者：
Popp, Juergen
中科院分区：
文献类型：
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作者：
Kloss, Sandra;Kampe, Bernd;Popp, Juergen
尿路感染（UTI）是一种非常常见的感染。多达每两个女人在她的一生中会经历至少一次UTI发作。鉴定感染性微生物的金标准是尿培养。然而，培养方法是耗时的，需要至少24小时，直到结果可用。在这里，我们报告了一个文化独立的识别程序，通过使用拉曼显微光谱结合创新的化学计量学。我们调查，第一次直接，尿液样品的拉曼显微光谱在单细胞水平上。在第一步中，建立了在无菌过滤尿液中生长的11种重要UTI细菌物种的数据库。一个支持向量机（SVM）被用来生成一个统计模型，该模型允许该数据集的分类，在物种水平上的准确度为92%。该模型随后用于直接鉴定10名患者的受感染尿液样本，而无需预先进行培养步骤。因此，我们能够确定所有10个患者样本的主要细菌种类（7种大肠杆菌和3种粪肠球菌）。这些结果表明，拉曼显微光谱与支持向量机的组合允许在两小时内鉴定重要的UTI细菌，而不需要培养步骤。
Urinary tract infection (UTI) is a very common infection. Up to every second woman will experience at least one UTI episode during her lifetime. The gold standard for identifying the infectious microorganisms is the urine culture. However, culture methods are time-consuming and need at least 24 h until the results are available. Here, we report about a culture independent identification procedure by using Raman microspectroscopy in combination with innovative chemo-metrics. We investigated, for the first time directly, urine samples by Raman microspectroscopy on a single-cell level. In a first step, a database of eleven important UTI bacterial species, which were grown in sterile filtered urine, was built up. A support vector machine (SVM) was used to generate a statistical model, which allows a classification of this data set with an accuracy of 92% on a species level. This model was afterward used to identify infected urine samples of ten patients directly without a preceding culture step. Thereby, we were able to determine the predominant bacterial species (seven Escherichia coli and three Enterococcus faecalis) for all ten patient samples. These results demonstrate that Raman microspectroscopy in combination with support vector machines allow an identification of important UTI bacteria within two hours without the need of a culture step.