Function of prolactin cells in the individual rat pituitary gland is location dependent
Function of prolactin cells in the individual rat pituitary gland is location dependent
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个体大鼠垂体中催乳素细胞的功能取决于位置
DOI：
10.1016/0303-7207(91)90257-s
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发表时间：
1991
影响因子：
4.1
通讯作者：
W. Hymer
中科院分区：
文献类型：
--
作者：
P. Mukherjee;T. Salada;W. Hymer
切除卵巢（ovx）或卵巢-雌激素（e2）治疗大鼠垂体前叶切片18块。每个切片连续孵育4个15 min，测定免疫反应性和生物活性催乳素（PRL）的释放；然后将每个切片胰化成单细胞悬液，以测定该切片中的PRL细胞数量。激素释放（ng PRL/100 PRL细胞）在整个腺体中不均匀；分泌模式的一致性表明PRL从腺体释放的量是位置依赖的。数据的统计分析表明，最活跃的细胞在腺体的左叶，而最不活跃的是在右叶。在这些叶中，左叶背-尾和腹-吻侧释放的激素最多，而右叶背-吻侧、背-尾和腹-吻侧释放的激素最少。给卵巢大鼠注射e2 2天后改变了这些分泌模式。这种切片方法在未来的研究中应该证明是有用的，因为它们与垂体细胞功能有关，可以解决细胞间相互作用和地理位置的问题。
Anterior pituitary glands from individual ovariectomized (ovx) or ovx-estrogen (E 2) treated rats were sectioned into 1 8 cubes. Each section was incubated for four consecutive 15 min periods in order to measure the release of immunoreactive and bioactive prolactin (PRL); each individual section was then trypsinized into a single cell suspension for determination of PRL cell numbers in that section. Hormone release (ng PRL/100 PRL cells) was not uniform throughout the gland; the consistency of the secretory patterns demonstrated that the amount of PRL release from the gland was location-dependent. Statistical analysis of the data showed that the most active cells were in the gland's left lobe, while the least active were in the right lobe. Within these lobes, the dorsal-caudal and ventral-rostral left lobe areas released the most hormone in vitro while those in the dorsal-rostral, dorsal-caudal and ventral-rostral right lobe areas were least active. Injection of ovx rats with E 2 for 2 days altered these secretory patterns. This sectioning procedure should prove useful in future studies addressing issues of cell-cell interaction and geographic location as they relate to pituitary cell function.
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新合成和储存的激素对生长激素和催乳素细胞基础分泌的相对重要性。
影响因子：
4.8
作者：
Chen,TT;Kineman,RD;Betts,JG;Hill,JB;Frawley,LS
通讯作者：
Frawley,LS
大鼠垂体前叶细胞的流式细胞术免疫荧光。
DOI：
10.1002/cyto.990060209
发表时间：
1985
期刊：
Cytometry
影响因子：
--
作者：
Hatfield,JM;Hymer,WC
通讯作者：
Hymer,WC
尽管老年雄性大鼠与年轻雄性大鼠的免疫反应性激素释放增加，但循环催乳素的生物活性降低。
影响因子：
4.8
作者：
Sylvester,PW;Briski,KP
通讯作者：
Briski,KP
新合成的催乳素颗粒的优先释放是促乳腺激素细胞功能异质性的结果。
影响因子：
4.8
作者：
Walker,AM;Farquhar,MG
通讯作者：
Farquhar,MG
Nb 2 淋巴结淋巴瘤细胞中催乳素依赖性有丝分裂：免疫抑制环肽的作用。
DOI：
--
发表时间：
1987
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Russell,DH;Buckley,AR;Montgomery,DW;Larson,NA;Gout,PW;Beer,CT;Putnam,CW;Zukoski,CF;Kibler,R
通讯作者：
Kibler,R