ENHANCED ACETYLCHOLINE-RELEASE IN HIPPOCAMPUS AND CORTEX DURING THE ANTICIPATION AND CONSUMPTION OF A PALATABLE MEAL
ENHANCED ACETYLCHOLINE-RELEASE IN HIPPOCAMPUS AND CORTEX DURING THE ANTICIPATION AND CONSUMPTION OF A PALATABLE MEAL
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DOI：
10.1016/0306-4522(94)90342-5
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发表时间：
1994-10-01
期刊：
影响因子：
3.3
通讯作者：
FIBIGER, HC
中科院分区：
文献类型：
--
作者：
INGLIS, FM;DAY, JC;FIBIGER, HC
在大鼠训练14天消耗可口的液体巧克力餐（Sustacal），在体内脑微透析被用来测量释放的乙酰胆碱在额叶皮层和海马在预期和消费的膳食。在实验室中训练大鼠，其中通过屏幕将大鼠与Sustacal分开20分钟（训练，奖励组）。然后移除屏障，并允许大鼠进食20分钟。同时运行两个对照组：这些组由以下大鼠组成：（i）接受了14天以上的训练，但只能在实验室中获得水（经过训练，无奖励），或（ii）在最终测试（即透析）阶段首次引入实验室，并给予Sustacal（幼稚）。在海马和额叶皮层中获得了不同的结果。在海马体中，乙酰胆碱的释放没有组间差异。因此，在所有三组乙酰胆碱释放增加到约220%的基础值时，动物被放置在实验室。在额叶皮层，乙酰胆碱的释放也在所有三组中显著增加。然而，这种增加的程度是显着更大的培训，奖励组，达到约300%的基础值在预期和完善的组成部分task.The乙酰胆碱释放的显着增加发生在海马和额叶皮层的三个组中的每一个是一致的参与胆碱能基底前脑神经元的调节唤醒或注意。此外，然而，在额叶皮层的乙酰胆碱释放可以进一步选择性地增强动物的过去的训练经验，也许是与预期的奖励。
In rats trained for 14 days to consume a palatable liquid chocolate meal (Sustacal), in vivo brain microdialysis was used to measure release of acetylcholine in the frontal cortex and hippocampus during anticipation and consumption of the meal. Rats were trained in an experimental chamber in which they were separated from the Sustacal by a screen for 20 min (trained, rewarded group). The screen was then removed and the rats were allowed 20 min of access to the meal. Two control groups were run concurrently: these groups consisted of rats (i) that were trained over 14 days but only had access to water in the experimental chamber (trained, non-rewarded), or (ii) that were introduced into the experimental chamber for the first time on the final test (i.e. dialysis) session, and presented with Sustacal (naive). Different results were obtained in the hippocampus and frontal cortex. In the hippocampus there were no group differences with respect to acetylcholine release. Thus, in all three groups acetylcholine release increased to about 220% of basal values when animals were placed in the experimental chamber. In the frontal cortex, acetylcholine release also increased significantly in all three groups. However, the extent of this increase was significantly greater in the trained, rewarded group, reaching approximately 300% of basal values during the anticipatory and consummatory components of the task.The significant increases in acetylcholine release which occurred in both the hippocampus and frontal cortex of each of the three groups are consistent with an involvement of cholinergic basal forebrain neurons in the regulation of arousal or attention. In addition, however, acetylcholine release in the frontal cortex can be further selectively enhanced by the animal's past training experience, perhaps being associated with the anticipation of reward.