Introgressive hybridization between two non-native apple snails in China: widespread hybridization and homogenization in egg morphology
Introgressive hybridization between two non-native apple snails in China: widespread hybridization and homogenization in egg morphology
复制标题
中国两种外来福寿螺的渐渗杂交：广泛的杂交和卵形态的同质化
DOI：
10.1002/ps.5980
复制
发表时间：
2020
影响因子：
4.1
通讯作者：
Yu Xiao-Ping
中科院分区：
文献类型：
--
作者：
Yang Qian-Qian;He Chao;Liu Guang-Fu;Yin Chuan-Lin;Xu Yi-Peng;Liu Su-Wen;Qiu Jian-Wen;Yu Xiao-Ping
BACKGROUNDApple snails from the genusPomaceahave spread widely in paddy fields and other wetlands of southern China since their introduction in the 1980s.Pomaceaspp. are commonly identified using mitochondrial COI sequences. However, sequencing the nuclear elongation factor 1‐alpha (EF1α) gene revealed genetic introgression between field populations ofP. canaliculataandP. maculata, which produce surviving hybrids in laboratory crossbreeding experiments.RESULTSIn this study, we sequenced 1054 EF1α clones to design specific primers and established a fast and accurate multiplex polymerase chain reaction (PCR) method for genotyping EF1α. Combined with genotypingP. canaliculataandP. maculatabased on mitochondrial COI and nuclear EF1α, we revealed the genetic introgression patterns of 30 apple snail populations in China. Purebred and hybrid individuals ofP. canaliculatawere widely distributed, while pure maculata‐EF1α type was detected only in a few individuals identified asP. canaliculatabased on COI sequences. Each egg clutch had one to three genetic patterns, indicating multiple paternity or segregation in the progeny of hybrids. The higher percentages of hybrids in both wild populations and progeny than the homozygotes indicated a potential heterosis in the apple snail populations. Additionally, egg size and clutch size of the apple snails became homogeneous among the non‐native populations exhibiting introgression hybridization.CONCLUSIONOur findings emphasize the value of apple snails as a model to study the mechanisms and impacts of introgressive hybridization on fitness traits. © 2020 Society of Chemical Industry
