Comparative permeability of canine visceral and parietal pleura.
Comparative permeability of canine visceral and parietal pleura.
复制标题
犬内脏和壁层胸膜的比较渗透性。
DOI：
10.1152/jappl.1988.65.6.2558
复制
发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Gonzalez,E
中科院分区：
文献类型：
--
作者：
Payne,DK;Kinasewitz,GT;Gonzalez,E
为了确定犬胸膜间皮的渗透性，将杂种狗的内脏和肋间壁胸膜从下面的组织中小心地剥离，并作为平面片安装在尤斯型室中。水力传导率 (Lp) 由响应于施加到胸膜粘膜或浆膜表面的静水压力梯度的体积通量速率确定。放射性标记的水、蔗糖、菊粉和白蛋白的扩散渗透性 (Pd) 在平衡条件下由单向示踪剂通量测定。脏层胸膜的Lp为0.39+/-0.032(SE)×10(-4)ml.s-1.cmH2O-1.cm-2，壁层胸膜的Lp为1.93+/-0.93×10(-4)ml.s-1.cmH2O-1.cm-2(P小于0.001)。脏层胸膜的 Pd 范围为 3H2O 的 12.21 +/- 0.45 X 10(-4) cm/s 到 [3H]白蛋白的 0.34 +/- 0.03 X 10(-4) cm/s。壁层胸膜对水和蔗糖的 Pd 与内脏膜相似，而对较大的菊粉和白蛋白分子的 Pd 大于内脏胸膜（P 小于 0.01）。跨膜均未检测到自发电位差。对于较大的溶质，相对较高的壁层胸膜 Lp 和 Pd 可能是由于该膜中存在气孔。这些结果表明，壁层胸膜和脏层胸膜都是渗透性极强的组织，对水和溶质通量的阻力很小。
To determine the permeability of canine pleural mesothelium, visceral and intercostal parietal pleura from mongrel dogs was carefully stripped from the underlying tissue and mounted as a planar sheet in a Ussing-type chamber. The hydraulic conductivity (Lp) was determined from the rate of volume flux in response to hydrostatic pressure gradients applied to either the mucosal or serosal surface of the pleural membrane. The diffusional permeability (Pd) of radiolabeled water, sucrose, inulin, and albumin was determined under equilibrium conditions from the unidirectional tracer flux. The Lp of the visceral pleura was 0.39 +/- 0.032 (SE) X 10(-4) ml.s-1.cmH2O-1.cm-2 and that Lp of parietal pleura was 1.93 +/- 0.93 X 10(-4) ml.s-1.cmH2O-1.cm-2 (P less than 0.001). The Pd of the visceral pleura ranged from 12.21 +/- 0.45 X 10(-4) cm/s for 3H2O to 0.34 +/- 0.03 X 10(-4) cm/s for [3H]albumin. The Pd of the parietal pleura for water and sucrose was similar to that of the visceral membrane, whereas its Pd for the larger inulin and albumin molecules was greater than that of visceral pleura (P less than 0.01). A spontaneous potential difference could not be detected across either membrane. The relatively higher parietal pleural Lp and Pd for larger solutes is probably due to the presence of stomata in this membrane. These results indicate that both the parietal and the visceral pleura are extremely permeable tissues which offer little resistance to water and solute flux.