Lhcb2 gene expression analysis in two ecotypes of Sedumalfredii subjected to Zn/Cd treatments with functional analysis of SaLhcb2 isolated from a Zn/Cd hyperaccumulator
Lhcb2 gene expression analysis in two ecotypes of Sedumalfredii subjected to Zn/Cd treatments with functional analysis of SaLhcb2 isolated from a Zn/Cd hyperaccumulator
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DOI：
10.1007/s10529-011-0622-8
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发表时间：
2011-04
影响因子：
2.7
通讯作者：
Min Zhang;T. Senoura;Xiaoe Yang;Y. Chao;N. Nishizawa
中科院分区：
文献类型：
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作者：
Min Zhang;T. Senoura;Xiaoe Yang;Y. Chao;N. Nishizawa
在不同浓度的Cd 2+处理24 h后，超积累植物东南景天Lhcb 2基因的表达量比对照植物高3倍以上，而非超积累植物的Lhcb 2基因的表达量比对照植物高1 ~ 2倍;非超积累植物东南景天在2 μM Cd 2+或50 μM Zn 2+处理8 d后，Lhcb 2基因的表达量比对照植物高5倍以上，而超积累植物的Lhcb 2基因的表达量比对照植物高2倍以上。比对照植物的mRNA丰度高一倍。过量表达SaLhcb 2可使地上部生物量增加14-41%，使根系生物量增加21-57%。4个转基因烟草株系（L5、L7、L10和L11）在Cd ~（2+）胁迫下地上部生物量均高于野生型植株。4个转基因株系（L7、L 8、L10和L11）地上部Cd ~（2+）积累量比野生型高6-35%。
TheLhcb2gene from hyperaccumulatorSedum alfrediiwas up-regulated more than three-fold while the non-hyperaccumulator accumulated one or two-fold higher amount of the mRNA than control plants under different concentrations of Cd2+for 24 h.Lhcb2expression was up-regulated more than five-fold in a non-hyperaccumulatorS.alfrediiwhen exposed to 2 μM Cd2+or 50 μM Zn2+for 8 d and the hyperaccumulator had over two-fold more mRNA abundance than the control plants. Over-expression ofSaLhcb2increased the shoot biomass by 14–41% and the root biomass by 21–57% without Cd2+treatment. Four transgenic tobacco lines (L5, L7, L10 and L11) possessed higher shoot biomass than WT plants with Cd2+. Four transgenic lines (L7, L8, L10 and L11) accumulated 6–35% higher Cd2+amounts in shoots than the wild type plants.