Mechanisms Underlying the Cardioprotection of YangXinDingJi Capsule against Myocardial Ischemia in Rats.
Mechanisms Underlying the Cardioprotection of YangXinDingJi Capsule against Myocardial Ischemia in Rats.
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DOI：
10.1155/2020/8539148
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Chu L
中科院分区：
文献类型：
--
作者：
Liu M;Xue Y;Liang Y;Xue Y;Han X;Li Z;Chu L
养心定基（YXDJ）胶囊是源自炙甘草汤的中药之一，在中国通常用于治疗心血管疾病。研究目的。心血管事件是全世界死亡的主要原因之一。心肌缺血（MI）严重缩短心肌细胞的寿命和功能。养心定基（YXDJ）胶囊在我国已用于治疗临床心脏病。然而，使用这种胶囊对心脏功能有益的潜在细胞机制仍不清楚。本研究的目的是评估YXDJ对异丙肾上腺素诱导的大鼠心肌梗死的保护作用，并阐明其基于L型钙通道和心肌收缩力的潜在心肌保护机制。 将大鼠随机分为5组，每组10只：（1）对照组； (2) ISO诱导模型； (3)高剂量YXDJ(2.8g/kg/天腹腔注射五天)，(4)低剂量YXDJ(1.4g/kg/天腹腔注射五天)； (5)维拉帕米(每组n = 10)。连续两天皮下注射异丙肾上腺素（ISO）诱导大鼠心肌梗死模型。获得心脏和生化参数。采用膜片钳技术观察YXDJ对离体心肌细胞L型钙电流(ICa-L)的调节作用。采用IonOptix MyoCam检测系统观察YXDJ对离体心肌细胞的收缩力。 YXDJ 显着改善病理心脏形态，减轻氧化应激和炎症反应。暴露于YXDJ导致ICa-L的阻断以浓度依赖性方式减少。 结果表明，YXDJ 显着抑制炎症细胞因子的表达、氧化应激和 L 型 Ca2+ 通道，并降低离体大鼠心肌细胞的收缩力。这些发现可能与 YXDJ 的心脏保护功效有关。
YangXinDingJi (YXDJ) capsule is one of traditional Chinese medicines (TCMs) derived from Zhigancao decoction, which is usually used for the treatment of cardiovascular disease in China. Aim of the Study. Cardiovascular events are one of the leading causes of death worldwide. Myocardial ischemia (MI) severely reduces myocyte longevity and function. The YangXinDingJi (YXDJ) capsule has been used in the treatment of clinical cardiac disease in China. Nevertheless, the underlying cellular mechanisms for the benefits to heart function resulting from the use of this capsule are still unclear. The aim of this study was to evaluate the protective effects of the YXDJ on isoprenaline-induced MI in rats and to clarify its underlying myocardial protective mechanisms based on L-type calcium channels and myocardial contractility. Rats were randomly divided into five groups with ten rats in each group: (1) control; (2) ISO-induced model; (3) high-dose YXDJ (2.8 g/kg/day intraperitoneally for five days), (4) low-dose YXDJ (1.4 g/kg/day for five days); and (5) verapamil (n = 10 in each group). Isoproterenol (ISO) was injected subcutaneously for two consecutive days to induce the rat model of MI. Heart and biochemical parameters were obtained. The patch-clamp technique was used to observe the regulatory effects of YXDJ on the L-type calcium current (ICa-L) in isolated cardiomyocytes. An IonOptix MyoCam detection system was used to observe the contractility of YXDJ on isolated cardiomyocytes. YXDJ caused a significant improvement in pathological heart morphology and alleviated oxidative stress and inflammatory responses. Exposure to YXDJ caused a decrease in blockade of ICa-L in a concentration-dependent manner. The results indicate that YXDJ significantly inhibited inflammatory cytokine expressions, oxidative stress, and L-type Ca2+ channels, and decreased contractility in isolated rat cardiomyocytes. These findings may be relevant to the cardioprotective efficacy of YXDJ.
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影响因子：
39.3
作者：
Aminzadeh, Mohammad A.;Tseliou, Eleni;Marban, Eduardo
通讯作者：
Marban, Eduardo
影响因子：
3.5
作者：
Annunziato, L;Amoroso, S;Di Renzo, GF
通讯作者：
Di Renzo, GF
影响因子：
7.2
作者：
Liu, Feng;Huang, Zhuang-Zhuang;Zhang, Tao
通讯作者：
Zhang, Tao
影响因子：
3.4
作者：
Greenstein, JL;Hinch, R;Winslow, RL
通讯作者：
Winslow, RL
瞬时和持续钙涌入的贡献，以及对去极化诱导的完整小鼠主动脉收缩的敏感性。
影响因子：
--
作者：
Fransen P;Van Hove CE;van Langen J;Schrijvers DM;Martinet W;De Meyer GR;Bult H
通讯作者：
Bult H