Silica-induced cytokine release from A549 cells: importance of surface area versus size
Silica-induced cytokine release from A549 cells: importance of surface area versus size
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DOI：
10.1191/096032701676225130
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发表时间：
2001-01-01
影响因子：
2.8
通讯作者：
Refsnes, M
中科院分区：
文献类型：
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作者：
Hetland, RB;Schwarze, PE;Refsnes, M
矿物颗粒的物理和化学性质，如结构、组成和表面反应性，决定了矿物颗粒的生物活性。众所周知，长期接触结晶二氧化硅会引起持续性肺部炎症，从而对健康产生不利影响。关于非晶二氧化硅对健康的潜在影响的信息较少。在这项研究中，评估了晶体和非晶态二氧化硅的炎症和细胞毒性与颗粒大小和表面积的关系。人上皮肺细胞（A549）暴露于不同大小的石英（气动直径0.5、2和10微米）和无定形二氧化硅（直径0.3微米）。所有颗粒均诱导促炎细胞因子白介素-6和IL-8的释放增加。当细胞暴露在等量的石英中时，最小的部分是最有效的。然而，当细胞因子释放与相同表面积相关时，这些差异消失。将无定形二氧化硅和石英进行比较，无论暴露方式如何表达，无定形二氧化硅诱导IL-6的能力最强，而最小尺寸的石英诱导IL-8的能力最强。因此，表面积似乎是关键的决定因素时，不同尺寸的石英的效力进行比较。
Physical and chemical properties such as structure, composition and surface reactivity determine the biological activity of mineral particles. Long-term exposure to crystalline silica is known to cause persistent pulmonary inflammation leading to adverse health effects. There is less information about the potential health effects of amorphous (noncrystalline) silica. In this study, the inflammatory and cytotoxic potency of crystalline and amorphous silica in relation to particle size and surface area was assessed. Human epithelial lung cells (A549) were exposed to different size fractions of quartz (aerodynamic diameter 0.5, 2 and 10 mum) and amorphous silica (diameter 0.3 mum). All particles induced increased release of the proinflammatory cytokines interleukin (IL)-6 and IL-8. When cells were exposed to equal masses of quartz, the smallest size fraction was the most potent. These differences, however, disappeared when cytokine release was related to equal surface areas. When amorphous silica and quartz were compared, the amorphous silica was most potent to induce IL-6 regardless of how exposure was expressed, whereas the smallest size fraction of quartz was the most potent inducer of IL-8. Thus, the surface area seems to be the critical determinant when potency of different sizes of quartz is compared.