A touch-actuated glucose sensor fully integrated with microneedle array and reverse iontophoresis for diabetes monitoring
A touch-actuated glucose sensor fully integrated with microneedle array and reverse iontophoresis for diabetes monitoring
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DOI：
10.1016/j.bios.2022.114026
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发表时间：
2022-02-01
影响因子：
12.6
通讯作者：
Jiang, Lelun
中科院分区：
文献类型：
--
作者：
Cheng, Yanxiang;Gong, Xia;Jiang, Lelun
由于传统的反向离子电渗法提取组织间液（ISF）存在提取通量低、一致性差等问题，因此，非侵入式组织间液葡萄糖生物传感器的开发仍具有挑战性。在这里，我们开发了一种触摸驱动的生物传感器，用于监测ISF中的葡萄糖。该生物传感器由三个主要组件组成：1）用于无痛皮肤穿透的固体微针阵列（MA）; 2）用于通过MA创建的微通道提取ISF的RI单元;以及3）用于葡萄糖监测的传感单元。该生物传感器的传感策略是“皮肤穿透-RI提取-电化学检测”。与仅RI提取相比，所报道的皮肤渗透-RI提取取样策略不仅在体外而且在体内都明显地增加了葡萄糖提取通量~1.6倍。此外，我们开发了一个可穿戴的葡萄糖监测系统，结合这种触摸驱动的生物传感器，无线电化学检测器，和智能手机应用程序。使用健康和糖尿病大鼠进行的体内实验显示，报告的可穿戴系统和商业血糖仪测量的结果之间具有高度相关性。这种结合皮肤渗透和RI提取的采样策略为开发可穿戴平台铺平了道路，不仅用于葡萄糖监测，还用于各种ISF生物标志物，而无需痛苦的手指针刺血液采样。
The development of non-invasive biosensor for monitoring glucose in interstitial fluid (ISF) is still challenging, because ISF extraction through classical reverse iontophoresis (RI) is limited by low extraction flux and consistency. Here, we developed a touch-actuated biosensor for monitoring glucose in ISF. The biosensor is composed of three main components: 1) the solid microneedle array (MA) for painless skin penetration; 2) the RI unit for ISF extraction through the MA-created microchannels; and 3) the sensing unit for glucose monitoring. The sensing strategy of this biosensor is "skin penetration-RI extraction-electrochemical detection ". Compared with RI extraction only, the reported skin penetration-RI extraction sampling strategy obviously increased the glucose extraction flux by ~1.6 times not only in vitro but also in vivo. Moreover, we developed a wearable glucose monitoring system by incorporating this touch-actuated biosensor, a wireless electrochemical detector, and a smartphone application. In vivo experiments using healthy and diabetic rats revealed a high correlation between the results measured by the reported wearable system and commercially blood glucometer. This sampling strategy which combined skin penetration and RI extraction paves the way to develop wearable platforms for not only glucose monitoring but also various ISF biomarkers without the need of painful finger-stick blood sampling.